caa a22 4500 378876015 CHVBK 20180305123420.0 cr unu---uuuuu 161128e20030515xx s 000 0 eng 10.1515/CCLM.2003.097 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.097 Early Detection of Peritonitis in ContinuousAmbulatory Peritoneal Dialysis Patients by CytokineMeasurements [Elektronische Daten] [Yüksel Oymak, Ayliz Velioğlu, Gonca Haklar, Çetin Özener, A. Süha Yalçin] Continuous ambulatory peritoneal dialysis (CAPD) carries a risk of peritonitis which is accompanied by mild symptomatology. Culture of effluent has yielded organism in 50% of cases. Peritoneal phagocytes produce tumor necrosis factor-α and interleukin (IL)-1 in response to contact with bacteria, initiating an inflammatory cascade which leads to IL-6 and IL-8 secretion. Additonally, neutrophils undergo an increase in oxidative metabolism. We have evaluated the diagnostic accuracy of effluent measurements of TNF-α, IL-6, IL-8, and oxidative metabolism markers in these patients. Dialysate fluids (n = 65) were collected from non-infected patients and those presenting with acute peritonitis. Positive culture proved the diagnosis. Oxidative markers and nitric oxide were measured by chemiluminescence. Cytokines were measured by solid phase chemiluminescent immunometric assay (Immulite, DPC, USA). Receiver operating characteristic (ROC) curves were used to assess the diagnostic accuracy and the areas under curves were calculated for comparison. All effluent cytokines and oxidative markers were significantly higher in patients with peritonitis when compared to those without (p < 0.05). Significant correlations were evident between IL-6 and IL-8, lucigenin chemiluminescence and luminol chemiluminescence, lucigenin chemiluminescence and IL-6 or IL-8, and luminol chemiluminescence and IL-6 or IL-8. ROC curves showed that the ability of IL-6, IL-8, lucigenin chemiluminescence, and luminol chemiluminescence to differentiate CAPD patients with peritonitis from non-infected cases exceeds that of polymorphonuclear leukocyte count. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Oymak Yüksel aut Velioğlu Ayliz aut Haklar Gonca aut Özener Çetin aut Yalçin A. Süha aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/5(2003-05-15), 640-645 1434-6621 41:5<640 2003 41 cclm https://doi.org/10.1515/CCLM.2003.097 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.097 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oymak Yüksel aut NATIONALLICENCE 700 1- Velioğlu Ayliz aut NATIONALLICENCE 700 1- Haklar Gonca aut NATIONALLICENCE 700 1- Özener Çetin aut NATIONALLICENCE 700 1- Yalçin A. Süha aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/5(2003-05-15), 640-645 1434-6621 41:5<640 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter