caa a22 4500 378876082 CHVBK 20180305123420.0 cr unu---uuuuu 161128e20030515xx s 000 0 eng 10.1515/CCLM.2003.096 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.096 Study of Apo(a) Length Polymorphism and Lipoprotein(a) Concentrations in Subjects with Single or Double Apo(a) Isoforms [Elektronische Daten] [Mariam Alaoui, Corinne Merle, Roselyne Garnotel, Damien Jolly, Vincent Durlach, Philippe Gillery] Cardiovascular risk is associated with high lipoprotein( a) (Lp(a)) concentrations and low molecular weight apolipoprotein(a) (apo(a)) isoforms. We studied the relationship between these two biological parameters, particularly in subjects expressing two apo(a) isoforms. Plasma Lp(a) was measured by immunonephelometry in 530 unrelated Caucasian patients at high cardiovascular risk, and apo(a) size determined by immunoblotting using a recombinant standard. Two, one, or no apo(a) isoforms were detected in 258, 270, and 2 subjects, respectively. Lp(a) concentrations showed a non-Gaussian distribution, being higher in the ‘double band' than in the ‘single band' group (median 0.42 vs. 0.11 g/l, p < 0.0005). Apo(a) size distribution was bimodal, with two frequency peaks at 18 kringles (K) and 27 K. Small size apo(a) isoforms were more frequently found in the ‘double band' group, where major isoforms were of lower size than minor isoforms (median 20 vs. 27 K). Regression analysis showed that apo(a) gene length accounted for 33% of Lp(a) variation, with a threshold effect at 20 K, no correlation being found over this value. The minor apo(a) isoform did not significantly influence Lp(a) concentration. These data confirm the relationship between apo(a) size and Lp(a) concentration and suggest that the assessment of cardiovascular risk should take into account the threshold effect at 20 K and the absence of influence of the minor apo(a) isoform. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Alaoui Mariam aut Merle Corinne aut Garnotel Roselyne aut Jolly Damien aut Durlach Vincent aut Gillery Philippe aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/5(2003-05-15), 634-639 1434-6621 41:5<634 2003 41 cclm https://doi.org/10.1515/CCLM.2003.096 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.096 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Alaoui Mariam aut NATIONALLICENCE 700 1- Merle Corinne aut NATIONALLICENCE 700 1- Garnotel Roselyne aut NATIONALLICENCE 700 1- Jolly Damien aut NATIONALLICENCE 700 1- Durlach Vincent aut NATIONALLICENCE 700 1- Gillery Philippe aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/5(2003-05-15), 634-639 1434-6621 41:5<634 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter