In vitro study of the interaction between neptunium ions and aposerumtransferrin by absorption spectrophotometry and ultrafiltration: the case of Np(V)
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 378877089 | ||
| 003 | CHVBK | ||
| 005 | 20180305123423.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 161128e20030201xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1524/ract.91.2.115.19987 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1524/ract.91.2.115.19987 | ||
| 245 | 0 | 0 | |a In vitro study of the interaction between neptunium ions and aposerumtransferrin by absorption spectrophotometry and ultrafiltration: the case of Np(V) |h [Elektronische Daten] |c [R. Racine, Ph. Moisy, F. Paquet, H. Métivier, Charles Madic] |
| 520 | 3 | |a The interaction between Np(V) and human aposerumtransferrin (apoTf) was studied in vitro under physiological conditions (37°C, pH 7.4, [NaCl]=0.15M, [HEPES]=5×10-2M) by UV-visible and near-IR absorption spectrophotometry and by ultrafiltration. It was found that Np(V) was bound in small fraction (<12%) to apoTf, and that the role of carbonate and citrate anions in binding is more competitive than synergistic. The complexes NpO2CO3- and NpO2Cit2- tend to form rather than the Np(V)-apoTf complex. Np(V) binding on apoTf appears to be pH-dependent and reversible. Displacement experiments with FeIIINTA showed non-specific binding, suggesting a weak interaction between Np(V) and apoTf different from the reactions involving transferrin-specific sites with other metallic ions, such as Fe(III). The low overall charge, the size and the geometry of the linear di-oxocation NpO2+ could account for the weak interaction between Np(V) and human transferrin. | |
| 540 | |a © 2003 Oldenbourg Wissenschaftsverlag GmbH | ||
| 690 | 7 | |a Laboratory techniques, experiments |2 nationallicence | |
| 690 | 7 | |a Analytical chemistry |2 nationallicence | |
| 690 | 7 | |a Inorganic chemistry |2 nationallicence | |
| 700 | 1 | |a Racine |D R. |4 aut | |
| 700 | 1 | |a Moisy |D Ph |4 aut | |
| 700 | 1 | |a Paquet |D F. |4 aut | |
| 700 | 1 | |a Métivier |D H. |4 aut | |
| 700 | 1 | |a Madic |D Charles |4 aut | |
| 773 | 0 | |t Radiochimica Acta/International journal for chemical aspects of nuclear science and technology |d Oldenbourg Wissenschaftsverlag GmbH |g 91/2/2003(2003-02-01), 115-122 |x 0033-8230 |q 91:2/2003<115 |1 2003 |2 91 |o ract | |
| 856 | 4 | 0 | |u https://doi.org/10.1524/ract.91.2.115.19987 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1524/ract.91.2.115.19987 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Racine |D R. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Moisy |D Ph |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Paquet |D F. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Métivier |D H. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Madic |D Charles |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Radiochimica Acta/International journal for chemical aspects of nuclear science and technology |d Oldenbourg Wissenschaftsverlag GmbH |g 91/2/2003(2003-02-01), 115-122 |x 0033-8230 |q 91:2/2003<115 |1 2003 |2 91 |o ract | ||
| 900 | 7 | |b CC0 |u http://creativecommons.org/publicdomain/zero/1.0 |2 nationallicence | |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-gruyter | ||