caa a22 4500 378877089 CHVBK 20180305123423.0 cr unu---uuuuu 161128e20030201xx s 000 0 eng 10.1524/ract.91.2.115.19987 doi (NATIONALLICENCE)gruyter-10.1524/ract.91.2.115.19987 In vitro study of the interaction between neptunium ions and aposerumtransferrin by absorption spectrophotometry and ultrafiltration: the case of Np(V) [Elektronische Daten] [R. Racine, Ph. Moisy, F. Paquet, H. Métivier, Charles Madic] The interaction between Np(V) and human aposerumtransferrin (apoTf) was studied in vitro under physiological conditions (37°C, pH 7.4, [NaCl]=0.15M, [HEPES]=5×10-2M) by UV-visible and near-IR absorption spectrophotometry and by ultrafiltration. It was found that Np(V) was bound in small fraction (<12%) to apoTf, and that the role of carbonate and citrate anions in binding is more competitive than synergistic. The complexes NpO2CO3- and NpO2Cit2- tend to form rather than the Np(V)-apoTf complex. Np(V) binding on apoTf appears to be pH-dependent and reversible. Displacement experiments with FeIIINTA showed non-specific binding, suggesting a weak interaction between Np(V) and apoTf different from the reactions involving transferrin-specific sites with other metallic ions, such as Fe(III). The low overall charge, the size and the geometry of the linear di-oxocation NpO2+ could account for the weak interaction between Np(V) and human transferrin. © 2003 Oldenbourg Wissenschaftsverlag GmbH Laboratory techniques, experiments nationallicence Analytical chemistry nationallicence Inorganic chemistry nationallicence Racine R. aut Moisy Ph aut Paquet F. aut Métivier H. aut Madic Charles aut Radiochimica Acta/International journal for chemical aspects of nuclear science and technology Oldenbourg Wissenschaftsverlag GmbH 91/2/2003(2003-02-01), 115-122 0033-8230 91:2/2003<115 2003 91 ract https://doi.org/10.1524/ract.91.2.115.19987 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1524/ract.91.2.115.19987 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Racine R. aut NATIONALLICENCE 700 1- Moisy Ph aut NATIONALLICENCE 700 1- Paquet F. aut NATIONALLICENCE 700 1- Métivier H. aut NATIONALLICENCE 700 1- Madic Charles aut NATIONALLICENCE 773 0- Radiochimica Acta/International journal for chemical aspects of nuclear science and technology Oldenbourg Wissenschaftsverlag GmbH 91/2/2003(2003-02-01), 115-122 0033-8230 91:2/2003<115 2003 91 ract CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter