Evaluation of ovotransferrin-matrix metal-affinity metalloprotein chromatography for Pu(IV) separations
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| 024 | 7 | 0 | |a 10.1524/ract.91.12.697.23418 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1524/ract.91.12.697.23418 | ||
| 245 | 0 | 0 | |a Evaluation of ovotransferrin-matrix metal-affinity metalloprotein chromatography for Pu(IV) separations |h [Elektronische Daten] |c [W. K. Myers, C. E. Ruggiero, S. D. Reilly, M. P. Neu] |
| 520 | 3 | |a Metal-Affinity metalloprotein chromatography has been suggested to have potential for selectively removing actinides from solution. To evaluate this potential, characterization of Pu(IV) and Fe(III) binding to an ovotransferrin-Sepharose™ 4B matrix, with oxalate and carbonate synergistic anions, was performed. Metal binding capacity was determined for metal concentrations ranging from 2×10-6 to 1×10-3M Fe(III) and 2.9×10-5 to 2.6×10-4M Pu(IV), where both metals were added as a solution of 10:1 nitrilotriacetic acid:metal. Metal binding capacity was also determined over the pH range 3.0 to 9.2. The metal binding properties of the immobilized ovotransferrin were found to differ from those reported for free-protein studies. Distribution coefficients were low: for metal solution concentrations of 0.02, 0.04, 0.10, and 0.20mM, coefficients were 540, 180, 23, and 12mL/g for Fe(III) and 20, 12, 7.2, and 6.0mL/g for Pu(IV), respectively. The capacity of the material was less dependent on pH than expected, varying by only two μmoles metal/g matrix for both Fe(III) and Pu(IV) over the pH range of 4.2 to 9.5. This study illustrates limitations of metalloprotein chromatography for Pu(IV) separation. | |
| 540 | |a © 2003 Oldenbourg Wissenschaftsverlag GmbH | ||
| 690 | 7 | |a Laboratory techniques, experiments |2 nationallicence | |
| 690 | 7 | |a Analytical chemistry |2 nationallicence | |
| 690 | 7 | |a Inorganic chemistry |2 nationallicence | |
| 700 | 1 | |a Myers |D W. K. |4 aut | |
| 700 | 1 | |a Ruggiero |D C. E. |4 aut | |
| 700 | 1 | |a Reilly |D S. D. |4 aut | |
| 700 | 1 | |a Neu |D M. P. |4 aut | |
| 773 | 0 | |t Radiochimica Acta/International journal for chemical aspects of nuclear science and technology |d Oldenbourg Wissenschaftsverlag GmbH |g 91/12/2003(2003-12-01), 697-704 |x 0033-8230 |q 91:12/2003<697 |1 2003 |2 91 |o ract | |
| 856 | 4 | 0 | |u https://doi.org/10.1524/ract.91.12.697.23418 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1524/ract.91.12.697.23418 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Myers |D W. K. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ruggiero |D C. E. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Reilly |D S. D. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Neu |D M. P. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Radiochimica Acta/International journal for chemical aspects of nuclear science and technology |d Oldenbourg Wissenschaftsverlag GmbH |g 91/12/2003(2003-12-01), 697-704 |x 0033-8230 |q 91:12/2003<697 |1 2003 |2 91 |o ract | ||
| 900 | 7 | |b CC0 |u http://creativecommons.org/publicdomain/zero/1.0 |2 nationallicence | |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-gruyter | ||