caa a22 4500 378878379 CHVBK 20180305123426.0 cr unu---uuuuu 161128e20031201xx s 000 0 eng 10.1524/ract.91.12.697.23418 doi (NATIONALLICENCE)gruyter-10.1524/ract.91.12.697.23418 Evaluation of ovotransferrin-matrix metal-affinity metalloprotein chromatography for Pu(IV) separations [Elektronische Daten] [W. K. Myers, C. E. Ruggiero, S. D. Reilly, M. P. Neu] Metal-Affinity metalloprotein chromatography has been suggested to have potential for selectively removing actinides from solution. To evaluate this potential, characterization of Pu(IV) and Fe(III) binding to an ovotransferrin-Sepharose™ 4B matrix, with oxalate and carbonate synergistic anions, was performed. Metal binding capacity was determined for metal concentrations ranging from 2×10-6 to 1×10-3M Fe(III) and 2.9×10-5 to 2.6×10-4M Pu(IV), where both metals were added as a solution of 10:1 nitrilotriacetic acid:metal. Metal binding capacity was also determined over the pH range 3.0 to 9.2. The metal binding properties of the immobilized ovotransferrin were found to differ from those reported for free-protein studies. Distribution coefficients were low: for metal solution concentrations of 0.02, 0.04, 0.10, and 0.20mM, coefficients were 540, 180, 23, and 12mL/g for Fe(III) and 20, 12, 7.2, and 6.0mL/g for Pu(IV), respectively. The capacity of the material was less dependent on pH than expected, varying by only two μmoles metal/g matrix for both Fe(III) and Pu(IV) over the pH range of 4.2 to 9.5. This study illustrates limitations of metalloprotein chromatography for Pu(IV) separation. © 2003 Oldenbourg Wissenschaftsverlag GmbH Laboratory techniques, experiments nationallicence Analytical chemistry nationallicence Inorganic chemistry nationallicence Myers W. K. aut Ruggiero C. E. aut Reilly S. D. aut Neu M. P. aut Radiochimica Acta/International journal for chemical aspects of nuclear science and technology Oldenbourg Wissenschaftsverlag GmbH 91/12/2003(2003-12-01), 697-704 0033-8230 91:12/2003<697 2003 91 ract https://doi.org/10.1524/ract.91.12.697.23418 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1524/ract.91.12.697.23418 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Myers W. K. aut NATIONALLICENCE 700 1- Ruggiero C. E. aut NATIONALLICENCE 700 1- Reilly S. D. aut NATIONALLICENCE 700 1- Neu M. P. aut NATIONALLICENCE 773 0- Radiochimica Acta/International journal for chemical aspects of nuclear science and technology Oldenbourg Wissenschaftsverlag GmbH 91/12/2003(2003-12-01), 697-704 0033-8230 91:12/2003<697 2003 91 ract CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter