caa a22 4500 378879219 CHVBK 20180305123428.0 cr unu---uuuuu 161128e20030721xx s 000 0 eng 10.1515/CCLM.2003.140 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.140 High-Throughput Liquid Chromatography-Tandem Mass Spectrometric Analysis ofSirolimus in Whole Blood [Elektronische Daten] [Pierre E. Wallemacq, Roger Vanbinst, Stéphanie Asta, Donald P. Cooper] Sirolimus appears as a new potent immunosuppressive agent taking advantage of therapeutic drug monitoring to optimize its use in organ transplantation. In the absence of any available commercial immunoassay it was mandatory to develop chromatographic assays. Some methods have already been proposed to quantify sirolimus in whole blood, based either on HPLC-UV, liquid chromatography-mass spectrometry (LC-MS) or liquid chromatography-tandem mass spectrometry (LC-MS/MS). We have developed a new faster and simpler LC-MS/MS method to quantify sirolimus in blood using ascomycin as an internal standard and multiple reaction monitoring (MRM) acquisition mode. This method displays a limit of detection of 0.3 μg/l, and the intra-assay reproducibility ranges from 4.1-7.9%. The pre-analytical preparation steps are quite similar to those required for semi-automated immunoassays. Ascomycin and sirolimus present retention times of 0.89 and 0.93 min, respectively, and the turnaround time for a result (2.5 min) is also similar to that observed using a clinical analyzer. The comparison performed between HPLC-UV and LC-MS/MS displays good correlation (r = 0.949). The LC-MS/MS method described above has been used routinely for more than 2000 patient blood specimens and may present several advantages over existing methods, e.g., specificity with sufficient sensitivity, rapidity, and small blood sampling (10 μl), making it particularly adapted for routine clinical use. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Wallemacq Pierre E. aut Vanbinst Roger aut Asta Stéphanie aut Cooper Donald P. aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/7(2003-07-21), 921-925 1434-6621 41:7<921 2003 41 cclm https://doi.org/10.1515/CCLM.2003.140 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.140 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wallemacq Pierre E. aut NATIONALLICENCE 700 1- Vanbinst Roger aut NATIONALLICENCE 700 1- Asta Stéphanie aut NATIONALLICENCE 700 1- Cooper Donald P. aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/7(2003-07-21), 921-925 1434-6621 41:7<921 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter