caa a22 4500 378881094 CHVBK 20180305123432.0 cr unu---uuuuu 161128e20030101xx s 000 0 eng 10.1351/pac200375070927 doi (NATIONALLICENCE)gruyter-10.1351/pac200375070927 Akasaka Kazuyuki Department of Biotechnological Science, School of Biology-Oriented Science and Technology, Kinki University, Wakayama 649-6493, Japan and Cellular Signaling Laboratory, RIKEN Harima Institute, Hyogo 679-5148, Japan Exploring the entire conformational space of proteins by high-pressure NMR [Elektronische Daten] [Kazuyuki Akasaka] A protein in solution is a thermodynamic entity, spanning, in principle, the entire allowed conformational space from the fully folded N to the fully unfolded U. Although some alternately or partially folded higher-energy conformers may coexist with N and U, they are seldom detected spectroscopically because their populations are usually quite low under physiological conditions. I describe here a new type of experiment, a combination of multidimensional NMR spectroscopy with pressure, that is capable of detecting and analyzing structures and thermodynamic stability of these higher-energy conformers. The idea is based on the finding that under physiological conditions the conformational order of a globular protein normally decreases in parallel with its partial molar volume (negative δV), so that under equilibrium conditions, the population is shifted to a less and-less-ordered conformer with increasing pressure. In principle, with the high space resolution of the multidimensional NMR, the method enables one to explore protein structure and stability in atomic detail in a wide conformational space from N to U with pressure and temperature as variables. The method will provide us with a strong basis for understanding the fundamental phenomena of proteins:function, folding, and aggregation. © 2013 Walter de Gruyter GmbH, Berlin/Boston Pure and Applied Chemistry De Gruyter 75/7(2003-01-01), 927-936 0033-4545 75:7<927 2003 75 pac https://doi.org/10.1351/pac200375070927 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1351/pac200375070927 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Akasaka Kazuyuki Department of Biotechnological Science, School of Biology-Oriented Science and Technology, Kinki University, Wakayama 649-6493, Japan and Cellular Signaling Laboratory, RIKEN Harima Institute, Hyogo 679-5148, Japan NATIONALLICENCE 773 0- Pure and Applied Chemistry De Gruyter 75/7(2003-01-01), 927-936 0033-4545 75:7<927 2003 75 pac CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter