caa a22 4500 378881132 CHVBK 20180305123432.0 cr unu---uuuuu 161128e20030101xx s 000 0 eng 10.1351/pac200375070859 doi (NATIONALLICENCE)gruyter-10.1351/pac200375070859 Prausnitz J. M. Chemical Engineering Department, University of California, Berkeley and Chemical Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA Molecular thermodynamics for some applications in biotechnology [Elektronische Daten] [J. M. Prausnitz] As biotechnology sweeps the world, it is appropriate to remember that the great virtue of thermodynamics is its broad range of applicability. As a result, there is a growing literature describing how chemical thermodynamics can be used to inform processes for old and new biochemical products for industry and medicine.A particular application of molecular thermodynamics concerns separation of aqueous proteins by selective precipitation. For this purpose, we need phase diagrams; for constructing such diagrams, we need to understand not only the qualitative nature of phase equilibria of aqueous proteins, but also the quantitative intermolecular forces between proteins in solution. Some examples are given to show how aqueous protein-protein forces can be calculated or measured to yield a potential of mean force and how that potential is then used along with a statistical-thermodynamic model to establish liquid -liquid and liquid -crystal equilibria. Such equilibria are useful not only for separation processes, but also for understanding diseases like Alzheimer's, eye cataracts, and sickle-cell anemia that appear to be caused by protein agglomeration. © 2013 Walter de Gruyter GmbH, Berlin/Boston Pure and Applied Chemistry De Gruyter 75/7(2003-01-01), 859-873 0033-4545 75:7<859 2003 75 pac https://doi.org/10.1351/pac200375070859 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1351/pac200375070859 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Prausnitz J. M. Chemical Engineering Department, University of California, Berkeley and Chemical Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA NATIONALLICENCE 773 0- Pure and Applied Chemistry De Gruyter 75/7(2003-01-01), 859-873 0033-4545 75:7<859 2003 75 pac CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter