caa a22 4500 378881434 CHVBK 20180305123432.0 cr unu---uuuuu 161128e20030807xx s 000 0 eng 10.1515/CCLM.2003.161 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.161 Stimulatory Effect of Homocysteine on Interleukin-8 Expression in Human EndothelialCells [Elektronische Daten] [Jürgen Geisel, Verena Jödden, Rima Obeid, Jean Pierre Knapp, Marion Bodis, Wolfgang Herrmann] Elevated plasma homocysteine is an independent risk factor for atherosclerosis. An important initial step of atherosclerosis is the adhesion and infiltration of monocytes to the lesion site. It has been shown that the pro-inflammatory cytokine interleukin-8 can rapidly cause rolling monocytes to adhere firmly onto monolayers expressing E-selectin. The objective of the present study was to investigate the effect of homocysteine on interleukin-8 production in human endothelial cells. Cells were incubated with various concentrations of homocysteine for 20 h. The gene expression was determined by real-time PCR and the interleukin-8 protein was measured by immunoassay analysis. Homocysteine enhanced the expression of interleukin-8 in a dose-dependent manner (181% of controls at 2.5 mmol/l homocysteine). Stimulation of gene expression was associated with a parallel increase in interleukin-8 protein synthesis (160% of controls at 5.0 mmol/l homocysteine). By co-incubation of endothelial cells with homocysteine and copper sulfate, a further elevation of interleukin-8 expression (251% of controls) was observed, whereas copper sulfate alone had no stimulatory effect. In conclusion, the present study demonstrated that homocysteine altered endothelial cell function by stimulating interleukin-8 expression, suggesting a contribution of homocysteine to the initiation and progression of atherosclerosis. The formation of homocysteine-induced oxidation products might serve as one of the underlying mechanisms of this effect. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Geisel Jürgen aut Jödden Verena aut Obeid Rima aut Knapp Jean Pierre aut Bodis Marion aut Herrmann Wolfgang aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/8(2003-08-07), 1045-1048 1434-6621 41:8<1045 2003 41 cclm https://doi.org/10.1515/CCLM.2003.161 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.161 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Geisel Jürgen aut NATIONALLICENCE 700 1- Jödden Verena aut NATIONALLICENCE 700 1- Obeid Rima aut NATIONALLICENCE 700 1- Knapp Jean Pierre aut NATIONALLICENCE 700 1- Bodis Marion aut NATIONALLICENCE 700 1- Herrmann Wolfgang aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/8(2003-08-07), 1045-1048 1434-6621 41:8<1045 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter