caa a22 4500 378881558 CHVBK 20180305123433.0 cr unu---uuuuu 161128e20030807xx s 000 0 eng 10.1515/CCLM.2003.160 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.160 Assay for Cross-Linked Carboxyterminal Telopeptide of Type I Collagen (ICTP) Unlike CrossLaps Assay Reflects Increased Pathological Degradation of Type I Collagen in Rheumatoid Arthritis [Elektronische Daten] [Mirja-Liisa Sassi, Sari Åman, Markku Hakala, Reijo Luukkainen, Juha Risteli] We compared the ability of assay for cross-linked carboxyterminal telopeptide of type I collagen (ICTP) and CrossLaps assay to reflect increased pathological degradation of type I collagen in serum and synovial fluid samples of patients with rheumatoid arthritis (RA; n = 40). ICTP and CrossLaps concentrations were correlated with each other and with markers of collagen synthesis (PINP and PIIINP, amino terminal propeptides of type I and type III procollagens, respectively) and with markers of inflammation, i.e., C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR). Serum ICTP was increased in half of the RA patients, whereas CrossLaps assays were increased only occasionally. Serum ICTP correlated with the other markers of collagen metabolism as well as with CRP and ESR. Serum CrossLaps correlated only with PINP and ICTP, but not with serum PIIINP, CRP or ESR. Two patients had false-positive reactions in the CrossLaps assay due to the rheumatoid factor. The ICTP and CrossLaps antigens were clearly separate peaks in gel filtration analysis. The CrossLaps assay is able to detect the same ICTP antigen, but not vice versa. The ICTP assay reflects increased matrix metalloproteinase-mediated collagen degradation in joints in RA. In contrast, the physiological cathepsin K-mediated bone resorption measured by the CrossLaps assay was only occasionally increased. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Sassi Mirja-Liisa aut Åman Sari aut Hakala Markku aut Luukkainen Reijo aut Risteli Juha aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/8(2003-08-07), 1038-1044 1434-6621 41:8<1038 2003 41 cclm https://doi.org/10.1515/CCLM.2003.160 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.160 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sassi Mirja-Liisa aut NATIONALLICENCE 700 1- Åman Sari aut NATIONALLICENCE 700 1- Hakala Markku aut NATIONALLICENCE 700 1- Luukkainen Reijo aut NATIONALLICENCE 700 1- Risteli Juha aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/8(2003-08-07), 1038-1044 1434-6621 41:8<1038 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter