caa a22 4500 378881566 CHVBK 20180305123433.0 cr unu---uuuuu 161128e20030807xx s 000 0 eng 10.1515/CCLM.2003.162 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.162 Sensitive and Specific Photometric Determination of Mannitol in Human Serum [Elektronische Daten] [Hendrik Graefe, Barbara Gütschow, Hartmut Gehring, Leif Dibbelt] Mannitol is an osmotically active polyalcohol often present in fluids used for irrigation of exposed tissue during minimal invasive surgery. Since this polyol normally is not detected in human plasma to any significant extent, it may be used as a laboratory marker of absorption of mannitol-containing irrigative fluids during surgery. For this aim, we developed a photometric assay of mannitol in human blood or serum that may be performed in a near-patient setting. Following deproteinization of the sample with trichloroacetic acid, the supernatant is mixed with NAD+ and a commercially available preparation of mannitol 2-dehydrogenase and is incubated at pH7.8 and at 37 °C for 30 to 60 minutes. At the end of the incubation period the solution is appropriately diluted and the concentration of NADH formed by oxidation of mannitol is determined photometrically at 340 nm. The limit of detection of serum mannitol with this assay is 0.05 mmol/l, the linear range of measurement extends to about 3 mmol/l. At analyte concentrations of 0.48, 1.38 and 3.48 mmol/l, coefficients of inter-assay variation of 12.1, 6.7 and 4.9%, respectively, were obtained. The analytical recovery of mannitol added to serum samples was close to 100%. Of 27 polyalcohols, monosaccharides and oligosaccharides tested, none exhibited a measurable substrate activity and only D-fructose significantly inhibited the oxidation of mannitol at sample concentrations above 10 mmol/l; the enzymatic reaction, however, was strongly affected by EDTA. The suitability of the assay as a routine diagnostic tool for detection and quantification of intraoperatively absorbed irrigation fluid was demonstrated by analyzing mannitol in serum samples obtained from 24 patients undergoing transurethral prostatectomy. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Graefe Hendrik aut Gütschow Barbara aut Gehring Hartmut aut Dibbelt Leif aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/8(2003-08-07), 1049-1055 1434-6621 41:8<1049 2003 41 cclm https://doi.org/10.1515/CCLM.2003.162 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.162 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Graefe Hendrik aut NATIONALLICENCE 700 1- Gütschow Barbara aut NATIONALLICENCE 700 1- Gehring Hartmut aut NATIONALLICENCE 700 1- Dibbelt Leif aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/8(2003-08-07), 1049-1055 1434-6621 41:8<1049 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter