caa a22 4500 378882678 CHVBK 20180305123435.0 cr unu---uuuuu 161128e20041104xx s 000 0 eng 10.1515/JPM.2004.128 doi (NATIONALLICENCE)gruyter-10.1515/JPM.2004.128 Detection of parvovirus B19, cytomegalovirus and enterovirus infections in cases of intrauterine fetal death [Elektronische Daten] [Karin Petersson, Oscar Norbeck, Magnus Westgren, Kristina Broliden] Aims: Maternal infections with parvovirus B19, cytomegalovirus (CMV) and enterovirus have been associated with intrauterine fetal death (IUFD), but the incidence of these infections is not clear. This prospective study was conducted to estimate this incidence. Methods: A prospective study of 38 months was conducted on cases of IUFD referred to Huddinge University Hospital, Stockholm, Sweden. Placental biopsies, fetal blood and amniotic fluid were collected from cases of IUFD (n=52). Placental biopsies from normal pregnancies at term (n=53) were used as controls. These tissues were examined for parvovirus B19 DNA, CMV DNA and enterovirus RNA using polymerase chain reaction (PCR). Maternal viral serology was measured in 46 cases and virus isolation for enterovirus in maternal stool samples was performed in 31 cases. Results: Viral nucleic acid was recovered in at least one tissue sample from six cases of fetal death (parvovirus B19 in two cases, CMV in three and enterovirus in one), while all placental biopsies from controls were found negative. Serological signs of primary maternal infection were found in two of the cases, and virus isolation for enterovirus was negative in all samples examined. Conclusion: Parvovirus B19, CMV and enterovirus may be considered as etiologic agents in cases of fetal death. PCR on placental and/or fetal tissue improves diagnostic accuracy for these infections. © Walter de Gruyter Human reproduction, growth & development nationallicence Gynaecology & obstetrics nationallicence Paediatric medicine nationallicence Cytomegalovirus nationallicence Enterovirus nationallicence Fetal death nationallicence Parvovirus B19 nationallicence Pregnancy nationallicence Stillbirth nationallicence Petersson Karin Department of Obstetrics and Gynecology, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden aut Norbeck Oscar Division of Clinical Virology, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden aut Westgren Magnus Department of Obstetrics and Gynecology, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden aut Broliden Kristina Division of Clinical Virology, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden aut Journal of Perinatal Medicine Walter de Gruyter 32/6(2004-11-04), 516-521 0300-5577 32:6<516 2004 32 jpme https://doi.org/10.1515/JPM.2004.128 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/JPM.2004.128 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Petersson Karin Department of Obstetrics and Gynecology, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden aut NATIONALLICENCE 700 1- Norbeck Oscar Division of Clinical Virology, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden aut NATIONALLICENCE 700 1- Westgren Magnus Department of Obstetrics and Gynecology, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden aut NATIONALLICENCE 700 1- Broliden Kristina Division of Clinical Virology, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden aut NATIONALLICENCE 773 0- Journal of Perinatal Medicine Walter de Gruyter 32/6(2004-11-04), 516-521 0300-5577 32:6<516 2004 32 jpme CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter