An apoptotic inducer, aralin, is a novel type II ribosome-inactivating protein from Aralia elata
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| 024 | 7 | 0 | |a 10.1515/BC.2004.107 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/BC.2004.107 | ||
| 245 | 0 | 3 | |a An apoptotic inducer, aralin, is a novel type II ribosome-inactivating protein from Aralia elata |h [Elektronische Daten] |c [Makoto Tomatsu, Toshiya Kondo, Takafumi Yoshikawa, Takashi Komeno, Naomi Adachi, Yasushi Kawasaki, Akira Ikuta, Fumio Tashiro] |
| 520 | 3 | |a We recently found that aralin, a novel cytotoxic protein consisting of two subunits, from Aralia elata selectively induces apoptosis in transformed cells as compared to normal cells. Here we report that aralin is a lectin specific for galactose (Gal) and its derivatives, and possesses RNA N-glycosidase activity as a new type II ribosome-inactivating protein (RIP). The RNA N-glycosidase activity of aralin was detected in cell-free and whole cell systems by the generation of an R-fragment from 28S rRNA. Coinciding with appearance of the R-fragment in aralin-treated cells, significant inhibition of protein synthesis was observed prior to the onset of apoptosis. Aralin-evoked cell death was efficiently repressed by the addition of Gal and its derivatives. Interestingly, melibiose preferentially protected normal cells from apoptosis as compared with transformed cells. Using rhodamine-coupled aralin, the aralin receptor could be clearly detected around the cell surface of transformed cells, but to a lesser extent on normal cells. Receptor binding was suppressed by Gal. These results indicate that aralin is incorporated into cells via its Gal-containing cell surface receptor and induces apoptosis through its RIP activity. Moreover, the expression level and/or structural changes of the aralin receptor may affect the sensitivity toward aralin. | |
| 540 | |a © Walter de Gruyter | ||
| 690 | 7 | |a Biochemistry |2 nationallicence | |
| 690 | 7 | |a Molecular biology |2 nationallicence | |
| 690 | 7 | |a Cellular biology |2 nationallicence | |
| 690 | 7 | |a apoptosis |2 nationallicence | |
| 690 | 7 | |a aralin |2 nationallicence | |
| 690 | 7 | |a cytotoxic protein |2 nationallicence | |
| 690 | 7 | |a lectin |2 nationallicence | |
| 690 | 7 | |a ribosome-inactivating protein (RIP) |2 nationallicence | |
| 690 | 7 | |a RNA N-glycosidase |2 nationallicence | |
| 700 | 1 | |a Tomatsu |D Makoto |u Akita Research Institute of Food and Brewing (ARIF), Arayamachi, Akita 010-1623, Japan |4 aut | |
| 700 | 1 | |a Kondo |D Toshiya |u School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo 108-8641, Japan |4 aut | |
| 700 | 1 | |a Yoshikawa |D Takafumi |u School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo 108-8641, Japan |4 aut | |
| 700 | 1 | |a Komeno |D Takashi |u Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan |4 aut | |
| 700 | 1 | |a Adachi |D Naomi |u Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan |4 aut | |
| 700 | 1 | |a Kawasaki |D Yasushi |u Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan |4 aut | |
| 700 | 1 | |a Ikuta |D Akira |u Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan |4 aut | |
| 700 | 1 | |a Tashiro |D Fumio |u Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan |4 aut | |
| 773 | 0 | |t Biological Chemistry |d Walter de Gruyter |g 385/9(2004-09-01), 819-827 |x 1431-6730 |q 385:9<819 |1 2004 |2 385 |o bchm | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/BC.2004.107 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/BC.2004.107 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tomatsu |D Makoto |u Akita Research Institute of Food and Brewing (ARIF), Arayamachi, Akita 010-1623, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kondo |D Toshiya |u School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo 108-8641, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yoshikawa |D Takafumi |u School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo 108-8641, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Komeno |D Takashi |u Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Adachi |D Naomi |u Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kawasaki |D Yasushi |u Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ikuta |D Akira |u Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tashiro |D Fumio |u Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biological Chemistry |d Walter de Gruyter |g 385/9(2004-09-01), 819-827 |x 1431-6730 |q 385:9<819 |1 2004 |2 385 |o bchm | ||
| 900 | 7 | |b CC0 |u http://creativecommons.org/publicdomain/zero/1.0 |2 nationallicence | |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-gruyter | ||