caa a22 4500 378883992 CHVBK 20180305123438.0 cr unu---uuuuu 161128e20040901xx s 000 0 eng 10.1515/BC.2004.107 doi (NATIONALLICENCE)gruyter-10.1515/BC.2004.107 An apoptotic inducer, aralin, is a novel type II ribosome-inactivating protein from Aralia elata [Elektronische Daten] [Makoto Tomatsu, Toshiya Kondo, Takafumi Yoshikawa, Takashi Komeno, Naomi Adachi, Yasushi Kawasaki, Akira Ikuta, Fumio Tashiro] We recently found that aralin, a novel cytotoxic protein consisting of two subunits, from Aralia elata selectively induces apoptosis in transformed cells as compared to normal cells. Here we report that aralin is a lectin specific for galactose (Gal) and its derivatives, and possesses RNA N-glycosidase activity as a new type II ribosome-inactivating protein (RIP). The RNA N-glycosidase activity of aralin was detected in cell-free and whole cell systems by the generation of an R-fragment from 28S rRNA. Coinciding with appearance of the R-fragment in aralin-treated cells, significant inhibition of protein synthesis was observed prior to the onset of apoptosis. Aralin-evoked cell death was efficiently repressed by the addition of Gal and its derivatives. Interestingly, melibiose preferentially protected normal cells from apoptosis as compared with transformed cells. Using rhodamine-coupled aralin, the aralin receptor could be clearly detected around the cell surface of transformed cells, but to a lesser extent on normal cells. Receptor binding was suppressed by Gal. These results indicate that aralin is incorporated into cells via its Gal-containing cell surface receptor and induces apoptosis through its RIP activity. Moreover, the expression level and/or structural changes of the aralin receptor may affect the sensitivity toward aralin. © Walter de Gruyter Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence apoptosis nationallicence aralin nationallicence cytotoxic protein nationallicence lectin nationallicence ribosome-inactivating protein (RIP) nationallicence RNA N-glycosidase nationallicence Tomatsu Makoto Akita Research Institute of Food and Brewing (ARIF), Arayamachi, Akita 010-1623, Japan aut Kondo Toshiya School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo 108-8641, Japan aut Yoshikawa Takafumi School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo 108-8641, Japan aut Komeno Takashi Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan aut Adachi Naomi Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan aut Kawasaki Yasushi Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan aut Ikuta Akira Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan aut Tashiro Fumio Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan aut Biological Chemistry Walter de Gruyter 385/9(2004-09-01), 819-827 1431-6730 385:9<819 2004 385 bchm https://doi.org/10.1515/BC.2004.107 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2004.107 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tomatsu Makoto Akita Research Institute of Food and Brewing (ARIF), Arayamachi, Akita 010-1623, Japan aut NATIONALLICENCE 700 1- Kondo Toshiya School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo 108-8641, Japan aut NATIONALLICENCE 700 1- Yoshikawa Takafumi School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo 108-8641, Japan aut NATIONALLICENCE 700 1- Komeno Takashi Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan aut NATIONALLICENCE 700 1- Adachi Naomi Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan aut NATIONALLICENCE 700 1- Kawasaki Yasushi Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan aut NATIONALLICENCE 700 1- Ikuta Akira Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan aut NATIONALLICENCE 700 1- Tashiro Fumio Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 385/9(2004-09-01), 819-827 1431-6730 385:9<819 2004 385 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter