caa a22 4500 378884018 CHVBK 20180305123438.0 cr unu---uuuuu 161128e20040901xx s 000 0 eng 10.1515/BC.2004.103 doi (NATIONALLICENCE)gruyter-10.1515/BC.2004.103 RNA interference by small hairpin RNAs synthesised under control of the human 7S K RNA promoter [Elektronische Daten] [Dorota Koper-Emde, Lutz Herrmann, Björn Sandrock, Bernd-Joachim Benecke] Small interfering RNAs (siRNAs) represent RNA duplexes of 21 nucleotides in length that inhibit gene expression. We have used the human gene-external 7S K RNA promoter for synthesis of short hairpin RNAs (shRNAs) which efficiently target human lamin mRNA via RNA interference (RNAi). Here we demonstrate that orientation of the target sequence within the shRNA construct is important for interference. Furthermore, effective interference also depends on the length and/or structure of the shRNA. Evidence is presented that the human 7S K promoter is more active in vivo than other gene-external promoters, such as the human U6 small nuclear RNA (snRNA) gene promoter. © Walter de Gruyter Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence gene silencing nationallicence lamin A/C nationallicence pol III transcription nationallicence siRNA nationallicence Koper-Emde Dorota Lehrstuhl für Biochemie I, Fakultät für Chemie, Ruhr-Universität Bochum, D-44780 Bochum, Germany aut Herrmann Lutz Lehrstuhl für Biochemie I, Fakultät für Chemie, Ruhr-Universität Bochum, D-44780 Bochum, Germany aut Sandrock Björn Lehrstuhl für Biochemie I, Fakultät für Chemie, Ruhr-Universität Bochum, D-44780 Bochum, Germany aut Benecke Bernd-Joachim Lehrstuhl für Biochemie I, Fakultät für Chemie, Ruhr-Universität Bochum, D-44780 Bochum, Germany aut Biological Chemistry Walter de Gruyter 385/9(2004-09-01), 791-794 1431-6730 385:9<791 2004 385 bchm https://doi.org/10.1515/BC.2004.103 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2004.103 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koper-Emde Dorota Lehrstuhl für Biochemie I, Fakultät für Chemie, Ruhr-Universität Bochum, D-44780 Bochum, Germany aut NATIONALLICENCE 700 1- Herrmann Lutz Lehrstuhl für Biochemie I, Fakultät für Chemie, Ruhr-Universität Bochum, D-44780 Bochum, Germany aut NATIONALLICENCE 700 1- Sandrock Björn Lehrstuhl für Biochemie I, Fakultät für Chemie, Ruhr-Universität Bochum, D-44780 Bochum, Germany aut NATIONALLICENCE 700 1- Benecke Bernd-Joachim Lehrstuhl für Biochemie I, Fakultät für Chemie, Ruhr-Universität Bochum, D-44780 Bochum, Germany aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 385/9(2004-09-01), 791-794 1431-6730 385:9<791 2004 385 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter