caa a22 4500 378884069 CHVBK 20180305123438.0 cr unu---uuuuu 161128e20040901xx s 000 0 eng 10.1515/BC.2004.111 doi (NATIONALLICENCE)gruyter-10.1515/BC.2004.111 Comprehensive analysis of metabolites in Corynebacterium glutamicum by gas chromatography/mass spectrometry [Elektronische Daten] [Sergey Strelkov, Mirko von Elstermann, Dietmar Schomburg] An analytical method based on gas chromatography/mass spectrometry was developed for metabolome investigation of Corynebacterium glutamicum. For the first time a fast method for metabolic screening that can be automated is described for this organism. More than 1000 compounds could be detected per experiment, ca. 330 of those showed a peak area significantly above background. Out of these 164 compounds were identified so far, representing derivatives of 121 different metabolites, which were quantified in one sample. In spite of the different chemical nature of metabolites and high matrix content, a measurement reproducibility in the range of 6% error was achieved. The application of this method for the analysis of the adaptation of C. glutamicum to different growth conditions is demonstrated. © Walter de Gruyter Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Corynebacterium glutamicum nationallicence gas chromatography nationallicence mass spectrometry nationallicence metabolic profiling nationallicence metabolite nationallicence metabolome nationallicence metabolomics nationallicence Strelkov Sergey Institute of Biochemistry, University of Cologne, Zülpicher Str. 47, D-50674 Cologne, Germany aut von Elstermann Mirko Institute of Biochemistry, University of Cologne, Zülpicher Str. 47, D-50674 Cologne, Germany aut Schomburg Dietmar Institute of Biochemistry, University of Cologne, Zülpicher Str. 47, D-50674 Cologne, Germany aut Biological Chemistry Walter de Gruyter 385/9(2004-09-01), 853-861 1431-6730 385:9<853 2004 385 bchm https://doi.org/10.1515/BC.2004.111 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2004.111 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Strelkov Sergey Institute of Biochemistry, University of Cologne, Zülpicher Str. 47, D-50674 Cologne, Germany aut NATIONALLICENCE 700 1- von Elstermann Mirko Institute of Biochemistry, University of Cologne, Zülpicher Str. 47, D-50674 Cologne, Germany aut NATIONALLICENCE 700 1- Schomburg Dietmar Institute of Biochemistry, University of Cologne, Zülpicher Str. 47, D-50674 Cologne, Germany aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 385/9(2004-09-01), 853-861 1431-6730 385:9<853 2004 385 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter