caa a22 4500 378890522 CHVBK 20180305123453.0 cr unu---uuuuu 161128e20040405xx s 000 0 eng 10.1515/CCLM.2004.076 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2004.076 Influence of blood collection in plastic vs. glass evacuated serum-separator tubes on hormone and tumour marker levels [Elektronische Daten] [Eva M. L. Smets, Josien E. Dijkstra-Lagemaat, Marinus A. Blankenstein] Introduction of preanalytical automation in our laboratory required the use of plastic blood collection tubes. Because of possible interference caused by adsorption of components to the plastic wall and because there is virtually no literature on this subject, we investigated the influence of collection of serum in plastic tubes on the results of nearly all our immunoassays for hormones and tumour markers. Blood from healthy volunteers was collected simultaneously in glass and plastic tubes, or sera prepared from blood collected in glass tubes were brought into the plastic tubes under investigation. Hormone and tumour marker levels were measured in the pairs thus obtained. Results were analysed using paired t-tests or Wilcoxon signed rank tests. We found small but statistically significant differences (p<0.05) between glass and plastic for free triiodothyronine, progesterone, prolactin, prostate-specific antigen and pregnancy-associated plasma protein-A. Non-significant trends (0.05<p<0.15) were seen with α-foetoprotein, androstenedione, BR (an assay for carbohydrate antigen 15.3), human chorionic gonadotropin and testosterone. Passing and Bablok regression analysis, however, revealed no clinically relevant differences (slopes ranged from 0.89 for progesterone to 1.07 for pregnancy-associated plasma protein-A, while intercepts were all small). We concluded that a switch to plastic blood collection tubes could occur without any implications for the interpretation of results. Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Smets Eva M. L. aut Dijkstra-Lagemaat Josien E. aut Blankenstein Marinus A. aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/4(2004-04-05), 435-439 1434-6621 42:4<435 2004 42 cclm https://doi.org/10.1515/CCLM.2004.076 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2004.076 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Smets Eva M. L. aut NATIONALLICENCE 700 1- Dijkstra-Lagemaat Josien E. aut NATIONALLICENCE 700 1- Blankenstein Marinus A. aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/4(2004-04-05), 435-439 1434-6621 42:4<435 2004 42 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter