caa a22 4500 378890530 CHVBK 20180305123453.0 cr unu---uuuuu 161128e20040405xx s 000 0 eng 10.1515/CCLM.2004.071 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2004.071 Cerebrospinal fluid tau and Aβ42 concentrations in healthy subjects: delineation of reference intervals and their limitations [Elektronische Daten] [Pierre R. Burkhard, Roxane Fournier, Bernadette Mermillod, Karl-Heinz Krause, Constantin Bouras, Irmgard Irminger] Many limitations and conflicting results have cast serious doubts on the validity of cerebrospinal fluid tau and Aβ42 levels for the biological diagnosis of Alzheimer's disease, particularly extreme variations of the reference limits found by unrelated groups as a consequence of different reference populations used. In this study, we addressed the issue of defining reference limits for cerebrospinal fluid tau and Aβ42 in healthy adult individuals. One hundred and five neurologically intact subjects were enrolled according to strict inclusion criteria, 10 of them with autopsy confirmation of brain integrity. All cerebrospinal fluid samples were similarly and optimally processed as were the dosage methods used and the statistical analyses performed. A robust correlation with age was demonstrated for Aβ42 but not for tau. For tau, we found that an upper cut-off value of 443 ng/l allowed 95% of the subjects to be correctly classified as normal. For Aβ42, a lower cut-off value of 90 ng/l allowed a correct classification of 90% of the subjects. However, a large variance of the reference values, partly explained by the potential contamination of the reference population with presymptomatic dementia patients, may limit the use of reference limits based on living subjects. We propose that the issue of defining reference limits for both cerebrospinal fluid tau and Aβ42 may ultimately be settled by studying large numbers of autopsy-proven neurologically intact individuals only. Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Burkhard Pierre R. aut Fournier Roxane aut Mermillod Bernadette aut Krause Karl-Heinz aut Bouras Constantin aut Irminger Irmgard aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/4(2004-04-05), 396-407 1434-6621 42:4<396 2004 42 cclm https://doi.org/10.1515/CCLM.2004.071 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2004.071 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Burkhard Pierre R. aut NATIONALLICENCE 700 1- Fournier Roxane aut NATIONALLICENCE 700 1- Mermillod Bernadette aut NATIONALLICENCE 700 1- Krause Karl-Heinz aut NATIONALLICENCE 700 1- Bouras Constantin aut NATIONALLICENCE 700 1- Irminger Irmgard aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/4(2004-04-05), 396-407 1434-6621 42:4<396 2004 42 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter