Impurities from polypropylene microcentrifuge tubes as a potential source of interference in simultaneous analysis of multiple lipid-soluble antioxidants by HPLC with electrochemical detection
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| 024 | 7 | 0 | |a 10.1515/CCLM.2004.070 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/CCLM.2004.070 | ||
| 245 | 0 | 0 | |a Impurities from polypropylene microcentrifuge tubes as a potential source of interference in simultaneous analysis of multiple lipid-soluble antioxidants by HPLC with electrochemical detection |h [Elektronische Daten] |c [Hsiu-Chuan Yen, Yi-Tzu Hsu] |
| 520 | 3 | |a High-performance liquid chromatography (HPLC) with electrochemical detection was used to analyze lipidsoluble antioxidants and micronutrients in plasma. Small amounts of plasma samples are often extracted in polypropylene (PP) microcentrifuge tubes before HPLC analysis due to its convenience. We therefore investigated the effect of impurities released from different PP tubes during extraction on the electrochemical detection of retinol, lutein, α-tocopherol, γ-tocopherol, retinyl palmitate, β-carotene and total coenzyme Q10 by an HPLC systemwith a coulometric array detector (CoulArray HPLC). An array of eight electrical potentials and gradient elution were applied. Human plasma or ultrapure water were extracted in four different PP tubes. Peaks at different retention times with varied amounts of unknown compounds were detected from different PP tubes. Although all those unknown peaks could be differentiated frompeaks of interest by the CoulArray HPLC, they caused busy chromatographs, and one peak had an overlap with the dominant peak of lutein. Moreover, that peak completely masked the lutein peak when only one oxidizing potential was applied. Our results suggest potential interference fromPP tubes on electrochemical detection especially when using only one electrical potential or having poor separation for these antioxidants if PP tubes are not carefully tested. | |
| 540 | |a Copyright © 2004 by Walter de Gruyter GmbH & Co. KG | ||
| 690 | 7 | |a Medical equipment & techniques |2 nationallicence | |
| 690 | 7 | |a Medical diagnosis |2 nationallicence | |
| 690 | 7 | |a Diseases & disorders |2 nationallicence | |
| 700 | 1 | |a Yen |D Hsiu-Chuan |4 aut | |
| 700 | 1 | |a Hsu |D Yi-Tzu |4 aut | |
| 773 | 0 | |t Clinical Chemistry and Laboratory Medicine |d Walter de Gruyter |g 42/4(2004-04-05), 390-395 |x 1434-6621 |q 42:4<390 |1 2004 |2 42 |o cclm | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/CCLM.2004.070 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/CCLM.2004.070 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yen |D Hsiu-Chuan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hsu |D Yi-Tzu |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Clinical Chemistry and Laboratory Medicine |d Walter de Gruyter |g 42/4(2004-04-05), 390-395 |x 1434-6621 |q 42:4<390 |1 2004 |2 42 |o cclm | ||
| 900 | 7 | |b CC0 |u http://creativecommons.org/publicdomain/zero/1.0 |2 nationallicence | |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-gruyter | ||