caa a22 4500 378890646 CHVBK 20180305123453.0 cr unu---uuuuu 161128e20040405xx s 000 0 eng 10.1515/CCLM.2004.067 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2004.067 Hultberg Björn Extracellular concentration of homocysteine in human cell lines is influenced by specific inhibitors of cyst(e)ine transport [Elektronische Daten] [Björn Hultberg] Despite the growing evidence that plasma homocysteine is a cardiovascular risk factor, the mechanism behind the vascular injuries is still unknown. Studies of the cellular uptake systems for homocysteine are scarce, but membrane transporters of cyst(e)ine seem to be involved. In the present study the cellular uptake of extracellular homocysteine in HeLa and hepatoma cell lines is investigated by using several different transport inhibitors for cellular uptake of cyst(e)ine. It is shown that systems A and Xc-are the main transport systems for homocysteine uptake in HeLa cells. It is also confirmed that the magnitude of homocysteine uptake in hepatoma cells is lower than in HeLa cells. However, in the presence of high amounts of extracellular homocysteine both cell types exhibited a high elimination of homocysteine, which was inhibited by the presence of inhibitors of systems A or Xc-. It is possible that there is normally a high turnover of homocysteine in cell cultures, which is not detected by occasional determinations of homocysteine concentrations. The complex pattern of homocysteine production, release, uptake and distribution between different cells in the body is important to examine further in order to possibly be able to modulate the elimination of homocysteine from circulation and thereby lower the riskof cardiovascular disease. Copyright (c) 2004 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/4(2004-04-05), 378-383 1434-6621 42:4<378 2004 42 cclm https://doi.org/10.1515/CCLM.2004.067 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2004.067 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Hultberg Björn NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/4(2004-04-05), 378-383 1434-6621 42:4<378 2004 42 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter