Identification of Serpula lacrymans and other decay fungi in construction timber by sequencing of ribosomal DNA - A practical approach

Verfasser / Beitragende:
[N. Högberg, C. J. Land]
Ort, Verlag, Jahr:
2004
Enthalten in:
Holzforschung, 58/2(2004-03-15), 199-204
Format:
Artikel (online)
ID: 378892037
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024 7 0 |a 10.1515/HF.2004.030  |2 doi 
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245 0 0 |a Identification of Serpula lacrymans and other decay fungi in construction timber by sequencing of ribosomal DNA - A practical approach  |h [Elektronische Daten]  |c [N. Högberg, C. J. Land] 
520 3 |a We have approached species identification of wood decay fungi in construction wood by means of sequencing ribosomal DNA. Sequencing of the Internal Transcribed Spacer (ITS), which includes the 5.8S rDNA, is straightforward and provides a basis for species identification. Identification was either by BLAST search on sequences in GenBank or phylogenetic analysis. A number of important wood decay fungi such as Serpula lacrymans, S. himantioides, Antrodia serialis, A. sinuosa, Gloeophyllum sepiarium, Fomitopsis pinicola, Resinicium bicolor and Junghuhnia collabens have been successfully identified from fruitbodies and directly from wood samples. Sequence variants were found within the species sampled including S. himantioides, the close relative of S. lacrymans. But, among 27 samples from fruitbodies and mycelium of S. lacrymans, unexpectedly, no sequence variation was detected. 
540 |a Copyright © 2004 by Walter de Gruyter GmbH & Co. KG 
690 7 |a Timber & wood processing  |2 nationallicence 
690 7 |a Biotechnology  |2 nationallicence 
690 7 |a Materials science  |2 nationallicence 
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