caa a22 4500 378892037 CHVBK 20180305123457.0 cr unu---uuuuu 161128e20040315xx s 000 0 eng 10.1515/HF.2004.030 doi (NATIONALLICENCE)gruyter-10.1515/HF.2004.030 Identification of Serpula lacrymans and other decay fungi in construction timber by sequencing of ribosomal DNA - A practical approach [Elektronische Daten] [N. Högberg, C. J. Land] We have approached species identification of wood decay fungi in construction wood by means of sequencing ribosomal DNA. Sequencing of the Internal Transcribed Spacer (ITS), which includes the 5.8S rDNA, is straightforward and provides a basis for species identification. Identification was either by BLAST search on sequences in GenBank or phylogenetic analysis. A number of important wood decay fungi such as Serpula lacrymans, S. himantioides, Antrodia serialis, A. sinuosa, Gloeophyllum sepiarium, Fomitopsis pinicola, Resinicium bicolor and Junghuhnia collabens have been successfully identified from fruitbodies and directly from wood samples. Sequence variants were found within the species sampled including S. himantioides, the close relative of S. lacrymans. But, among 27 samples from fruitbodies and mycelium of S. lacrymans, unexpectedly, no sequence variation was detected. Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Timber & wood processing nationallicence Biotechnology nationallicence Materials science nationallicence Högberg N. aut Land C. J. aut Holzforschung Walter de Gruyter 58/2(2004-03-15), 199-204 0018-3830 58:2<199 2004 58 hfsg https://doi.org/10.1515/HF.2004.030 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/HF.2004.030 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Högberg N. aut NATIONALLICENCE 700 1- Land C. J. aut NATIONALLICENCE 773 0- Holzforschung Walter de Gruyter 58/2(2004-03-15), 199-204 0018-3830 58:2<199 2004 58 hfsg CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter