caa a22 4500 378892169 CHVBK 20180305123457.0 cr unu---uuuuu 161128e20040510xx s 000 0 eng 10.1515/CCLM.2004.085 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2004.085 Up-regulation of reactive oxygen species (ROS) and resistance to Fas-mediated apoptosis in the C33A cervical cancer cell line transfected with IL-18 receptor [Elektronische Daten] [Do-Young Yoon, Young-Sik Cho, Joo-Won Park, Soo-Hyun Kim, Jong-Wan Kim] Cervical cancer cells were transfected with a newly discovered interleukin (IL)-18 receptor to investigate the effect of endogenous IL-18 on the regulation of immune-related factors such as Fas (CD95/Apo-1)/Fas ligand and intercellular adhesion molecules. Transfection of the IL-18 receptor selectively induced a slight enhancement of the Fas via the up-regulation of intracellular reactive oxygen species and IL-18 in cervical carcinoma C33A cells, whereas there were no effects on the expression of p53, intercellular adhesion molecules-1 and Fas ligand. Neither IL-18 receptor transfection nor recombinant IL-18 enhanced interferon-γ production in C33A cells. Thus, IL-18 receptor transfection induced IL-18 expression and enhanced intracellular reactive oxygen species and Fas expression in C33A cells in an interferon-γ-independent pathway. However, treatment with agonistic anti-Fas antibody did not induce the apoptosis of C33A/IL-18 receptor transfectants, suggesting that either reactive oxygen species play a key role in resisting the Fas-induced apoptosis of C33A cells, or Fas was not functional. These results show that C33A/IL-18 receptor cells are resistant to the apoptosis and thus can survive against the immune surveillance and activated immune cells. Our results thus suggest that IL-18 and IL-18 receptor, together, may play a role in immunoregulation or in inflammation by augmenting the levels of IL-18 and reactive oxygen species in C33A cells. Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Yoon Do-Young aut Cho Young-Sik aut Park Joo-Won aut Kim Soo-Hyun aut Kim Jong-Wan aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/5(2004-05-10), 499-506 1434-6621 42:5<499 2004 42 cclm https://doi.org/10.1515/CCLM.2004.085 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2004.085 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yoon Do-Young aut NATIONALLICENCE 700 1- Cho Young-Sik aut NATIONALLICENCE 700 1- Park Joo-Won aut NATIONALLICENCE 700 1- Kim Soo-Hyun aut NATIONALLICENCE 700 1- Kim Jong-Wan aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/5(2004-05-10), 499-506 1434-6621 42:5<499 2004 42 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter