An International Standard for whole blood folate: evaluation of a lyophilised haemolysate in an international collaborative study
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| 024 | 7 | 0 | |a 10.1515/CCLM.2004.090 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/CCLM.2004.090 | ||
| 245 | 0 | 3 | |a An International Standard for whole blood folate: evaluation of a lyophilised haemolysate in an international collaborative study |h [Elektronische Daten] |c [Susan J. Thorpe, Dawn Sands, Alan B. Heath, Malcolm S. Hamilton, Sheena Blackmore, Trevor Barrowcliffe] |
| 520 | 3 | |a Folate measurements, particularly for whole blood, show wide inter-laboratory and inter-methodology variability. This variability appears to be due in part to the lack of internationally accepted reference materials. A whole blood haemolysate, lyophilised in ampoules and designated 95/528, was therefore evaluated by 15 laboratories in five countries for its suitability as an International Standard (IS) for whole blood folate. The preparation was assayed using a variety of microbiological and protein-binding methodologies against local standards and calibrators. A consensus folate content was assigned to 95/528. The inclusion of three whole blood samples in the study with widely differing folate levels demonstrated a considerable reduction in inter-laboratory variability when the folate content of the samples was determined relative to the proposed IS 95/528 rather than to laboratories' local standards and calibrators. Accelerated degradation studies indicated that the folate content of 95/528 is stable when stored at −20°C. On the basis of the results presented here, the World Health Organization Expert Committee on Biological Standardization established 95/528 as an IS for whole blood folate. | |
| 540 | |a Copyright © 2004 by Walter de Gruyter GmbH & Co. KG | ||
| 690 | 7 | |a Medical equipment & techniques |2 nationallicence | |
| 690 | 7 | |a Medical diagnosis |2 nationallicence | |
| 690 | 7 | |a Diseases & disorders |2 nationallicence | |
| 700 | 1 | |a Thorpe |D Susan J. |4 aut | |
| 700 | 1 | |a Sands |D Dawn |4 aut | |
| 700 | 1 | |a Heath |D Alan B. |4 aut | |
| 700 | 1 | |a Hamilton |D Malcolm S. |4 aut | |
| 700 | 1 | |a Blackmore |D Sheena |4 aut | |
| 700 | 1 | |a Barrowcliffe |D Trevor |4 aut | |
| 773 | 0 | |t Clinical Chemistry and Laboratory Medicine |d Walter de Gruyter |g 42/5(2004-05-10), 533-539 |x 1434-6621 |q 42:5<533 |1 2004 |2 42 |o cclm | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/CCLM.2004.090 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/CCLM.2004.090 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Thorpe |D Susan J. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sands |D Dawn |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Heath |D Alan B. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hamilton |D Malcolm S. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Blackmore |D Sheena |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Barrowcliffe |D Trevor |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Clinical Chemistry and Laboratory Medicine |d Walter de Gruyter |g 42/5(2004-05-10), 533-539 |x 1434-6621 |q 42:5<533 |1 2004 |2 42 |o cclm | ||
| 900 | 7 | |b CC0 |u http://creativecommons.org/publicdomain/zero/1.0 |2 nationallicence | |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-gruyter | ||