caa a22 4500 378892193 CHVBK 20180305123457.0 cr unu---uuuuu 161128e20040510xx s 000 0 eng 10.1515/CCLM.2004.090 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2004.090 An International Standard for whole blood folate: evaluation of a lyophilised haemolysate in an international collaborative study [Elektronische Daten] [Susan J. Thorpe, Dawn Sands, Alan B. Heath, Malcolm S. Hamilton, Sheena Blackmore, Trevor Barrowcliffe] Folate measurements, particularly for whole blood, show wide inter-laboratory and inter-methodology variability. This variability appears to be due in part to the lack of internationally accepted reference materials. A whole blood haemolysate, lyophilised in ampoules and designated 95/528, was therefore evaluated by 15 laboratories in five countries for its suitability as an International Standard (IS) for whole blood folate. The preparation was assayed using a variety of microbiological and protein-binding methodologies against local standards and calibrators. A consensus folate content was assigned to 95/528. The inclusion of three whole blood samples in the study with widely differing folate levels demonstrated a considerable reduction in inter-laboratory variability when the folate content of the samples was determined relative to the proposed IS 95/528 rather than to laboratories' local standards and calibrators. Accelerated degradation studies indicated that the folate content of 95/528 is stable when stored at −20°C. On the basis of the results presented here, the World Health Organization Expert Committee on Biological Standardization established 95/528 as an IS for whole blood folate. Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Thorpe Susan J. aut Sands Dawn aut Heath Alan B. aut Hamilton Malcolm S. aut Blackmore Sheena aut Barrowcliffe Trevor aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/5(2004-05-10), 533-539 1434-6621 42:5<533 2004 42 cclm https://doi.org/10.1515/CCLM.2004.090 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2004.090 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Thorpe Susan J. aut NATIONALLICENCE 700 1- Sands Dawn aut NATIONALLICENCE 700 1- Heath Alan B. aut NATIONALLICENCE 700 1- Hamilton Malcolm S. aut NATIONALLICENCE 700 1- Blackmore Sheena aut NATIONALLICENCE 700 1- Barrowcliffe Trevor aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/5(2004-05-10), 533-539 1434-6621 42:5<533 2004 42 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter