caa a22 4500 378892274 CHVBK 20180305123457.0 cr unu---uuuuu 161128e20040510xx s 000 0 eng 10.1515/CCLM.2004.083 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2004.083 Analysis of candidate genes in Polish families with obesity [Elektronische Daten] [Malgorzata Malczewska-Malec, Iwona Wybranska, Iwona Leszczynska-Golabek, Lukasz Partyka, Jadwiga Hartwich, Agata Jabrocka, Beata Kiec-Wilk, Malgorzata Kwasniak, Marcin Motyka, Aldona Dembinska-Kiec] This study analyzes the relationship between risk factors related to overweight/obesity, insulin resistance, lipid tolerance, hypertension, endothelial function and genetic polymorphisms associated with: i) appetite regulation (leptin, melanocortin-3-receptor (MCR-3), dopamine receptor 2 (D2R)); ii) adipocyte differentiation and insulin sensitivity (peroxisome proliferator-activated receptor-γ2 (PPAR-γ2), tumor necrosis factor-α (TNF-α)); iii) thermogenesis and free fatty acid (FFA) transport/catabolism (uncoupling protein-1 (UCP1), lipoprotein lipase (LPL), β2- and β3-adrenergic receptor (β2AR, β3AR), fatty acid transport protein-1 (FATP-1) and iv) lipoproteins (apoliprotein E (apoE), apo CIII). The 122 members of 40 obese Caucasian families from southern Poland participated in the study. The genotypes were analyzed by restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) or by direct sequencing. Phenotypes related to obesity (body mass index (BMI), fat/lean body mass composition, waist-to-hip ratio (WHR)), fasting lipids, glucose, leptin and insulin, as well as insulin during oral glucose tolerance test (OGTT) (4 points within 2 hours) and during oral lipid tolerance test (OLTT) (5 points within 8 hours) were assessed. The insulin sensitivity indexes: homeostasis model assessment of insulin resistance, whole body insulin sensitivity index, hepatic insulin sensitivity and early secretory response to an oral glucose load (HOMA-IR, ISI-COMP, ISI-HOMA and DELTA) were calculated. The single gene mutations such as C105 T OB and Pro115 Gln PPAR-γ2 linked to morbid obesity were not detected in our group. A weak correlation between obesity and certain gene polymorphisms was observed. Being overweight (25<BMI≤30 kg/m2) significantly correlated with worse FFA tolerance in male PPAR-γ2 12Pro, LPL-H (G) allele carriers. Insulin resistance was found in female PPAR-γ2 Pro12, TNF-α (−308A) and LPL-H (G) allele carriers. Hypertension linked to the PPAR-γ2 Pro allele carriers was characterized by high leptin output during OLTT. We conclude that the polymorphisms we investigated were weakly correlated with obesity but significantly modified the risk factors of the metabolic syndrome. Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Malczewska-Malec Malgorzata aut Wybranska Iwona aut Leszczynska-Golabek Iwona aut Partyka Lukasz aut Hartwich Jadwiga aut Jabrocka Agata aut Kiec-Wilk Beata aut Kwasniak Malgorzata aut Motyka Marcin aut Dembinska-Kiec Aldona aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/5(2004-05-10), 487-493 1434-6621 42:5<487 2004 42 cclm https://doi.org/10.1515/CCLM.2004.083 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2004.083 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Malczewska-Malec Malgorzata aut NATIONALLICENCE 700 1- Wybranska Iwona aut NATIONALLICENCE 700 1- Leszczynska-Golabek Iwona aut NATIONALLICENCE 700 1- Partyka Lukasz aut NATIONALLICENCE 700 1- Hartwich Jadwiga aut NATIONALLICENCE 700 1- Jabrocka Agata aut NATIONALLICENCE 700 1- Kiec-Wilk Beata aut NATIONALLICENCE 700 1- Kwasniak Malgorzata aut NATIONALLICENCE 700 1- Motyka Marcin aut NATIONALLICENCE 700 1- Dembinska-Kiec Aldona aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/5(2004-05-10), 487-493 1434-6621 42:5<487 2004 42 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter