caa a22 4500 378892290 CHVBK 20180305123457.0 cr unu---uuuuu 161128e20040510xx s 000 0 eng 10.1515/CCLM.2004.088 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2004.088 Plasma levels of 7-hydroxylated dehydroepiandrosterone (DHEA) metabolites and selected amino-thiols as discriminatory tools of Alzheimer's disease and vascular dementia [Elektronische Daten] [Marie Bičíková, Daniela Řípová, Martin Hill, Roman Jirák, Helena Havlíková, Jaroslava Tallová, Richard Hampl] Objective: The early differential diagnosis of Alzheimer's disease (AD) remains still problematic. We developed a laboratory test enabling us to distinguish patients with AD from those with vascular dementia (VD) and healthy subjects. Methods: The AD group consisted of 22 women and 18 men. The VD group consisted of 16 women and 8 men. Age-matched controls consisted of 12 women and 9 men. Plasma pregnenolone sulfate (PregS), dehydroepiandrosterone (DHEA) and its sulfate (DHEAS) were determined by radioimmunoassay. 17-Hydroxypregnenolone (17 Preg) and 7-hydroxylated metabolites of DHEA (7αDHEA, 7βDHEA) were determined by radioimmunoassay after separation by high performance liquid chromatography (HPLC). Homocysteine (Hcy), cysteine (Cys), cysteinylglycine (Cysgly) and glutathione (GSH) were measured by HPLC. Results: The ANOVA results of significant between-group differences were as follows: The PregS and the 17-Preg and DHEAS levels were independent from the diagnosis. The 7αDHEA levels significantly depended on the sex (p<0.05) and diagnosis (p<0.01). Aminothiols were influenced by the diagnosis (p<0.01, p=0.0541, p<0.01 and p=0.0536 for Cys, Hcy, Cysgly and GSH, respectively). Using a stepwise backward regression analysis, the following parameters were obtained: X=11.5+4.03×sex+1.09× Hcy+0.190 × PregS−4.76×DHEAS+3.00×DHEA− 34.3×7αDHEA−0.885× Cysgly from which P-value as a discriminator was calculated according to the formula: P=1/(1+e−x). Then, for P>0.5, a subject was considered as AD-positive (with 89% correct prediction). Discussion: The opportunity of early differential diagnosis of AD should help physicians to use suitable treatment for retardation of pathological processes. Copyright (c) 2004 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Bičíková Marie aut Řípová Daniela aut Hill Martin aut Jirák Roman aut Havlíková Helena aut Tallová Jaroslava aut Hampl Richard aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/5(2004-05-10), 518-524 1434-6621 42:5<518 2004 42 cclm https://doi.org/10.1515/CCLM.2004.088 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2004.088 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bičíková Marie aut NATIONALLICENCE 700 1- Řípová Daniela aut NATIONALLICENCE 700 1- Hill Martin aut NATIONALLICENCE 700 1- Jirák Roman aut NATIONALLICENCE 700 1- Havlíková Helena aut NATIONALLICENCE 700 1- Tallová Jaroslava aut NATIONALLICENCE 700 1- Hampl Richard aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/5(2004-05-10), 518-524 1434-6621 42:5<518 2004 42 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter