caa a22 4500 378893106 CHVBK 20180305123500.0 cr unu---uuuuu 161128e20040205xx s 000 0 eng 10.1515/BC.2004.036 doi (NATIONALLICENCE)gruyter-10.1515/BC.2004.036 Biochemical analysis of neutral endopeptidase activity reveals independent catabolism of atrial and brain natriuretic peptide [Elektronische Daten] [T. Walther, H. Stepan, K. Pankow, M. Becker, H.-P. Schultheiss, W.-E. Siems] Recent reports presented contradictory results regarding the catabolism of mature atrial (ANP) and brain (BNP) natriuretic peptides in circulation. Especially the role of neutral endopeptidase (NEP) in BNP degradation was conversely discussed. Our present in vitro-studies characterize the NEP-dependent metabolism of ANP and BNP in different tissues via HPLC-analysis using NEP-deficient mice and specific NEP inhibitors. Our results show a strong tissue-dependent degradation pattern of both peptides, which are not only due to the different NEP activities in these tissues. Whereas NEP rapidly degraded ANP, it had no influence in BNP-metabolism. Additional experiments with purified NEP confirmed this result. Moreover, we describe a degradation of ANP and BNP in NEP-deficient and NEP-inhibited membranes. Consequently, we postulate the existence of at least one further natriuretic peptide (NP) degrading enzyme, which has not been characterized yet. Thus, the commonly accepted model of the natriuretic peptide system with NEP as the central degrading peptidase has to be partly revised. Moreover, the NEP-independent BNP degradation provides an effective means for achieving a beneficial BNP increase in cardiovascular pathology by inhibiting the assumed novel NP-degrading peptidase(s). Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Walther T. aut Stepan H. aut Pankow K. aut Becker M. aut Schultheiss H.-P aut Siems W.-E aut Biological Chemistry Walter de Gruyter 385/2(2004-02-05), 179-184 1431-6730 385:2<179 2004 385 bchm https://doi.org/10.1515/BC.2004.036 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2004.036 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Walther T. aut NATIONALLICENCE 700 1- Stepan H. aut NATIONALLICENCE 700 1- Pankow K. aut NATIONALLICENCE 700 1- Becker M. aut NATIONALLICENCE 700 1- Schultheiss H.-P aut NATIONALLICENCE 700 1- Siems W.-E aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 385/2(2004-02-05), 179-184 1431-6730 385:2<179 2004 385 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter