The periplasmic E. coli chaperone Skp is a trimer in solution: biophysical and preliminary crystallographic characterization
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| 024 | 7 | 0 | |a 10.1515/BC.2004.032 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/BC.2004.032 | ||
| 245 | 0 | 4 | |a The periplasmic E. coli chaperone Skp is a trimer in solution: biophysical and preliminary crystallographic characterization |h [Elektronische Daten] |c [M. Schlapschy, M. K. Dommel, K. Hadian, M. Fogarasi, I.P. Korndörfer, A. Skerra] |
| 520 | 3 | |a The 'seventeen kilodalton protein' Skp confers transient solubility on outer membrane proteins during biogenesis in Gram-negative bacteria. Here we report a first biophysical characterization of this chaperone itself, which also possesses biotechnological potential in the production of recombinant proteins. Using cross-linking and gel filtration methods, we found that Skp forms a stable homo-trimer in solution. Following thermal denaturation, monitored by CD spectroscopy, this chaperone refolds with high efficiency but exhibits a pronounced hysteresis between the un- and refolding transitions. Using the recombinant protein equipped with the Strep-tag II at its N-terminus, suitable crystallization conditions for Skp were found. A first data set was collected to 2.60 å resolution. | |
| 540 | |a Copyright © 2004 by Walter de Gruyter GmbH & Co. KG | ||
| 690 | 7 | |a Biochemistry |2 nationallicence | |
| 690 | 7 | |a Molecular biology |2 nationallicence | |
| 690 | 7 | |a Cellular biology |2 nationallicence | |
| 700 | 1 | |a Schlapschy |D M. |4 aut | |
| 700 | 1 | |a Dommel |D M. K. |4 aut | |
| 700 | 1 | |a Hadian |D K. |4 aut | |
| 700 | 1 | |a Fogarasi |D M. |4 aut | |
| 700 | 1 | |a Korndörfer |D I.P. |4 aut | |
| 700 | 1 | |a Skerra |D A. |4 aut | |
| 773 | 0 | |t Biological Chemistry |d Walter de Gruyter |g 385/2(2004-02-05), 137-143 |x 1431-6730 |q 385:2<137 |1 2004 |2 385 |o bchm | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/BC.2004.032 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/BC.2004.032 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Schlapschy |D M. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Dommel |D M. K. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hadian |D K. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fogarasi |D M. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Korndörfer |D I.P. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Skerra |D A. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biological Chemistry |d Walter de Gruyter |g 385/2(2004-02-05), 137-143 |x 1431-6730 |q 385:2<137 |1 2004 |2 385 |o bchm | ||
| 900 | 7 | |b CC0 |u http://creativecommons.org/publicdomain/zero/1.0 |2 nationallicence | |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-gruyter | ||