First report on a potato I family chymotrypsin inhibitor from the seeds of a Cucurbitaceous plant, Momordica cochinchinensis
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| 024 | 7 | 0 | |a 10.1515/BC.2004.037 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/BC.2004.037 | ||
| 245 | 0 | 0 | |a First report on a potato I family chymotrypsin inhibitor from the seeds of a Cucurbitaceous plant, Momordica cochinchinensis |h [Elektronische Daten] |c [A. Y. K. Tsoi, R. C. H. Wong, T.-B. Ng, W.-P. Fong] |
| 520 | 3 | |a A 7514-Da chymotrypsin inhibitor was isolated from the seed extract of Momordica cochinchinensis (Family Cucurbitaceae) by chromatography on chymotrypsin Sepharose 4B and subsequently by C18 reversed-phase HPLC. This inhibitor, named MCoCI, possessed remarkable thermostability and was stable from pH 2 to 12. MCoCI also inhibited subtilisin, but had at least 50-fold lower inhibitory activity towards trypsin and elastase. Amino acid sequencing of a peptide fragment of MCoCI revealed a sequence of 23 amino acids. Comparison of this sequence and the molecular mass with those of other protease inhibitors suggests that MCoCI belongs to the potato I inhibitor family. | |
| 540 | |a Copyright © 2004 by Walter de Gruyter GmbH & Co. KG | ||
| 690 | 7 | |a Biochemistry |2 nationallicence | |
| 690 | 7 | |a Molecular biology |2 nationallicence | |
| 690 | 7 | |a Cellular biology |2 nationallicence | |
| 700 | 1 | |a Tsoi |D A. Y. K. |4 aut | |
| 700 | 1 | |a Wong |D R. C. H. |4 aut | |
| 700 | 1 | |a Ng |D T.-B |4 aut | |
| 700 | 1 | |a Fong |D W.-P |4 aut | |
| 773 | 0 | |t Biological Chemistry |d Walter de Gruyter |g 385/2(2004-02-05), 185-189 |x 1431-6730 |q 385:2<185 |1 2004 |2 385 |o bchm | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/BC.2004.037 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/BC.2004.037 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tsoi |D A. Y. K. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wong |D R. C. H. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ng |D T.-B |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fong |D W.-P |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biological Chemistry |d Walter de Gruyter |g 385/2(2004-02-05), 185-189 |x 1431-6730 |q 385:2<185 |1 2004 |2 385 |o bchm | ||
| 900 | 7 | |b CC0 |u http://creativecommons.org/publicdomain/zero/1.0 |2 nationallicence | |
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| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-gruyter | ||