caa a22 4500 378893157 CHVBK 20180305123500.0 cr unu---uuuuu 161128e20040205xx s 000 0 eng 10.1515/BC.2004.037 doi (NATIONALLICENCE)gruyter-10.1515/BC.2004.037 First report on a potato I family chymotrypsin inhibitor from the seeds of a Cucurbitaceous plant, Momordica cochinchinensis [Elektronische Daten] [A. Y. K. Tsoi, R. C. H. Wong, T.-B. Ng, W.-P. Fong] A 7514-Da chymotrypsin inhibitor was isolated from the seed extract of Momordica cochinchinensis (Family Cucurbitaceae) by chromatography on chymotrypsin Sepharose 4B and subsequently by C18 reversed-phase HPLC. This inhibitor, named MCoCI, possessed remarkable thermostability and was stable from pH 2 to 12. MCoCI also inhibited subtilisin, but had at least 50-fold lower inhibitory activity towards trypsin and elastase. Amino acid sequencing of a peptide fragment of MCoCI revealed a sequence of 23 amino acids. Comparison of this sequence and the molecular mass with those of other protease inhibitors suggests that MCoCI belongs to the potato I inhibitor family. Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Tsoi A. Y. K. aut Wong R. C. H. aut Ng T.-B aut Fong W.-P aut Biological Chemistry Walter de Gruyter 385/2(2004-02-05), 185-189 1431-6730 385:2<185 2004 385 bchm https://doi.org/10.1515/BC.2004.037 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2004.037 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tsoi A. Y. K. aut NATIONALLICENCE 700 1- Wong R. C. H. aut NATIONALLICENCE 700 1- Ng T.-B aut NATIONALLICENCE 700 1- Fong W.-P aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 385/2(2004-02-05), 185-189 1431-6730 385:2<185 2004 385 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter