Structural characterization of extracellular lipase from Streptomyces rimosus: assignment of disulfide bridge pattern by mass spectrometry
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| 008 | 161128e20041201xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1515/BC.2004.148 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/BC.2004.148 | ||
| 245 | 0 | 0 | |a Structural characterization of extracellular lipase from Streptomyces rimosus: assignment of disulfide bridge pattern by mass spectrometry |h [Elektronische Daten] |c [Ivana Leščić, Martin Zehl, Roland Müller, Bojana Vukelić, Marija Abramić, Jasenka Pigac, Günter Allmaier, Biserka Kojić-Prodić] |
| 520 | 3 | |a The cloning, sequencing and high-level expression of the gene encoding extracellular lipase from Streptomyces rimosus R6-554W have been recently described, and the primary structure of this gene product was deduced using a bioinformatic approach. In this study, capillary electrophoresis-on-the-chip and mass spectrometry were used to characterize native and overexpressed extracellular lipase protein from S. rimosus. The exact molecular mass of the wild-type and the overexpressed lipase, determined by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, were in excellent agreement (Δm=0.11Da and Δm=0.26Da, respectively) with a value of 24165.76Da calculated from the structure deduced from the nucleotide sequence, considering the mature enzyme with all six cysteines forming disulfide bridges. The primary structure derived from the nucleotide sequence was completely verified using a combination of tryptic digestion and formic acid cleavage of the protein, followed by peptide mass fingerprinting. Selected peptides were further investigated by MALDI low-energy collision-induced dissociation hybrid tandem mass spectrometry, allowing the unambiguous determination of their predicted amino acid sequence. No post-translational modifications of mature S. rimosus lipase were detected. Comparison of the peptide mass fingerprints from the reduced and non-reduced overexpressed enzyme unequivocally revealed three intramolecular disulfide bonds with the following linkages: C27-C52, C93-C101 and C151-C198. | |
| 540 | |a ©2004 by Walter de Gruyter Berlin New York | ||
| 690 | 7 | |a Biochemistry |2 nationallicence | |
| 690 | 7 | |a Molecular biology |2 nationallicence | |
| 690 | 7 | |a Cellular biology |2 nationallicence | |
| 690 | 7 | |a amino acid sequence |2 nationallicence | |
| 690 | 7 | |a capillary electrophoresis-on-the-chip |2 nationallicence | |
| 690 | 7 | |a GDSL lipolytic enzyme |2 nationallicence | |
| 690 | 7 | |a low-energy collision-induced dissociation |2 nationallicence | |
| 690 | 7 | |a matrix-assisted laser desorption/ionization |2 nationallicence | |
| 690 | 7 | |a streptomycetes |2 nationallicence | |
| 700 | 1 | |a Leščić |D Ivana |u Department of Physical Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, HR-10002 Zagreb, Croatia |4 aut | |
| 700 | 1 | |a Zehl |D Martin |u Institute of Chemical Technologies and Analysis, Vienna University of Technology, Getreidemarkt 9/164-IAC, A-1060 Vienna, Austria |4 aut | |
| 700 | 1 | |a Müller |D Roland |u Institute of Chemical Technologies and Analysis, Vienna University of Technology, Getreidemarkt 9/164-IAC, A-1060 Vienna, Austria |4 aut | |
| 700 | 1 | |a Vukelić |D Bojana |u Department of Organic Chemistry and Biochemistry, Ruđer Bošković Institute, Bijenička cesta 54, HR-10002 Zagreb, Croatia |4 aut | |
| 700 | 1 | |a Abramić |D Marija |u Department of Organic Chemistry and Biochemistry, Ruđer Bošković Institute, Bijenička cesta 54, HR-10002 Zagreb, Croatia |4 aut | |
| 700 | 1 | |a Pigac |D Jasenka |u Department of Physical Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, HR-10002 Zagreb, Croatia |4 aut | |
| 700 | 1 | |a Allmaier |D Günter |u Institute of Chemical Technologies and Analysis, Vienna University of Technology, Getreidemarkt 9/164-IAC, A-1060 Vienna, Austria |4 aut | |
| 700 | 1 | |a Kojić-Prodić |D Biserka |u Department of Physical Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, HR-10002 Zagreb, Croatia |4 aut | |
| 773 | 0 | |t Biological Chemistry |d Walter de Gruyter |g 385/12(2004-12-01), 1147-1156 |x 1431-6730 |q 385:12<1147 |1 2004 |2 385 |o bchm | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/BC.2004.148 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/BC.2004.148 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Leščić |D Ivana |u Department of Physical Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, HR-10002 Zagreb, Croatia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zehl |D Martin |u Institute of Chemical Technologies and Analysis, Vienna University of Technology, Getreidemarkt 9/164-IAC, A-1060 Vienna, Austria |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Müller |D Roland |u Institute of Chemical Technologies and Analysis, Vienna University of Technology, Getreidemarkt 9/164-IAC, A-1060 Vienna, Austria |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Vukelić |D Bojana |u Department of Organic Chemistry and Biochemistry, Ruđer Bošković Institute, Bijenička cesta 54, HR-10002 Zagreb, Croatia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Abramić |D Marija |u Department of Organic Chemistry and Biochemistry, Ruđer Bošković Institute, Bijenička cesta 54, HR-10002 Zagreb, Croatia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Pigac |D Jasenka |u Department of Physical Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, HR-10002 Zagreb, Croatia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Allmaier |D Günter |u Institute of Chemical Technologies and Analysis, Vienna University of Technology, Getreidemarkt 9/164-IAC, A-1060 Vienna, Austria |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kojić-Prodić |D Biserka |u Department of Physical Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, HR-10002 Zagreb, Croatia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biological Chemistry |d Walter de Gruyter |g 385/12(2004-12-01), 1147-1156 |x 1431-6730 |q 385:12<1147 |1 2004 |2 385 |o bchm | ||
| 900 | 7 | |b CC0 |u http://creativecommons.org/publicdomain/zero/1.0 |2 nationallicence | |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-gruyter | ||