Conserved asparagine residue 54 of α-sarcin plays a role in protein stability and enzyme activity
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| 003 | CHVBK | ||
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| 008 | 161128e20041201xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1515/BC.2004.150 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/BC.2004.150 | ||
| 245 | 0 | 0 | |a Conserved asparagine residue 54 of α-sarcin plays a role in protein stability and enzyme activity |h [Elektronische Daten] |c [Ansgar Siemer, Manuel Masip, Nelson Carreras, Lucía García-Ortega, Mercedes Oñaderra, Marta Bruix, Álvaro Martínez del Pozo, José G. Gavilanes] |
| 520 | 3 | |a Asparagine 54 of α-sarcin is a conserved residue within the proteins of the ribotoxin family of microbial ribonucleases. It is located in loop 2 of the protein, which lacks repetitive secondary structure elements but exhibits a well-defined conformation. Five mutant variants at this residue have been produced and characterized. The spectroscopic characterization of these proteins indicates that the overall conformation is not changed upon mutation. Activity and denaturation assays show that Asn-54 largely contributes to protein stability, and its presence is a requirement for the highly specific inhibitory activity of these ribotoxins on ribosomes. | |
| 540 | |a ©2004 by Walter de Gruyter Berlin New York | ||
| 690 | 7 | |a Biochemistry |2 nationallicence | |
| 690 | 7 | |a Molecular biology |2 nationallicence | |
| 690 | 7 | |a Cellular biology |2 nationallicence | |
| 690 | 7 | |a α-sarcin |2 nationallicence | |
| 690 | 7 | |a protein stability |2 nationallicence | |
| 690 | 7 | |a ribonuclease |2 nationallicence | |
| 690 | 7 | |a RNase T1 |2 nationallicence | |
| 700 | 1 | |a Siemer |D Ansgar |u Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain |4 aut | |
| 700 | 1 | |a Masip |D Manuel |u Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain |4 aut | |
| 700 | 1 | |a Carreras |D Nelson |u Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain |4 aut | |
| 700 | 1 | |a García-Ortega |D Lucía |u Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain |4 aut | |
| 700 | 1 | |a Oñaderra |D Mercedes |u Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain |4 aut | |
| 700 | 1 | |a Bruix |D Marta |u Instituto de Química-Física Rocasolano, CSIC, Serrano 119, E-28006 Madrid, Spain |4 aut | |
| 700 | 1 | |a Martínez del Pozo |D Álvaro |u Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain |4 aut | |
| 700 | 1 | |a G. Gavilanes |D José |u Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain |4 aut | |
| 773 | 0 | |t Biological Chemistry |d Walter de Gruyter |g 385/12(2004-12-01), 1165-1170 |x 1431-6730 |q 385:12<1165 |1 2004 |2 385 |o bchm | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/BC.2004.150 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/BC.2004.150 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Siemer |D Ansgar |u Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Masip |D Manuel |u Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Carreras |D Nelson |u Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a García-Ortega |D Lucía |u Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Oñaderra |D Mercedes |u Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bruix |D Marta |u Instituto de Química-Física Rocasolano, CSIC, Serrano 119, E-28006 Madrid, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Martínez del Pozo |D Álvaro |u Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a G. Gavilanes |D José |u Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biological Chemistry |d Walter de Gruyter |g 385/12(2004-12-01), 1165-1170 |x 1431-6730 |q 385:12<1165 |1 2004 |2 385 |o bchm | ||
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