caa a22 4500 378896008 CHVBK 20180305123506.0 cr unu---uuuuu 161128e20041201xx s 000 0 eng 10.1515/BC.2004.150 doi (NATIONALLICENCE)gruyter-10.1515/BC.2004.150 Conserved asparagine residue 54 of α-sarcin plays a role in protein stability and enzyme activity [Elektronische Daten] [Ansgar Siemer, Manuel Masip, Nelson Carreras, Lucía García-Ortega, Mercedes Oñaderra, Marta Bruix, Álvaro Martínez del Pozo, José G. Gavilanes] Asparagine 54 of α-sarcin is a conserved residue within the proteins of the ribotoxin family of microbial ribonucleases. It is located in loop 2 of the protein, which lacks repetitive secondary structure elements but exhibits a well-defined conformation. Five mutant variants at this residue have been produced and characterized. The spectroscopic characterization of these proteins indicates that the overall conformation is not changed upon mutation. Activity and denaturation assays show that Asn-54 largely contributes to protein stability, and its presence is a requirement for the highly specific inhibitory activity of these ribotoxins on ribosomes. ©2004 by Walter de Gruyter Berlin New York Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence α-sarcin nationallicence protein stability nationallicence ribonuclease nationallicence RNase T1 nationallicence Siemer Ansgar Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain aut Masip Manuel Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain aut Carreras Nelson Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain aut García-Ortega Lucía Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain aut Oñaderra Mercedes Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain aut Bruix Marta Instituto de Química-Física Rocasolano, CSIC, Serrano 119, E-28006 Madrid, Spain aut Martínez del Pozo Álvaro Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain aut G. Gavilanes José Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain aut Biological Chemistry Walter de Gruyter 385/12(2004-12-01), 1165-1170 1431-6730 385:12<1165 2004 385 bchm https://doi.org/10.1515/BC.2004.150 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2004.150 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Siemer Ansgar Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain aut NATIONALLICENCE 700 1- Masip Manuel Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain aut NATIONALLICENCE 700 1- Carreras Nelson Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain aut NATIONALLICENCE 700 1- García-Ortega Lucía Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain aut NATIONALLICENCE 700 1- Oñaderra Mercedes Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain aut NATIONALLICENCE 700 1- Bruix Marta Instituto de Química-Física Rocasolano, CSIC, Serrano 119, E-28006 Madrid, Spain aut NATIONALLICENCE 700 1- Martínez del Pozo Álvaro Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain aut NATIONALLICENCE 700 1- G. Gavilanes José Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040 Madrid, Spain aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 385/12(2004-12-01), 1165-1170 1431-6730 385:12<1165 2004 385 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter