Nuclear fibroblast growth factor-2 interacts specifically with splicing factor SF3a66
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| 024 | 7 | 0 | |a 10.1515/BC.2004.156 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/BC.2004.156 | ||
| 245 | 0 | 0 | |a Nuclear fibroblast growth factor-2 interacts specifically with splicing factor SF3a66 |h [Elektronische Daten] |c [Susanne Gringel, Jeroen van Bergeijk, Kirsten Haastert, Claudia Grothe, Peter Claus] |
| 520 | 3 | |a Fibroblast growth factor 2 (FGF-2) has a dual role as a classical extracellular signaling protein and as an intracellular factor. Isoforms of FGF-2, resulting from alternatively used start codons on one mRNA species, locate differentially to nuclear compartments. In this study we aimed to analyze functions of intracellular FGF-2 by identification of interacting proteins. We identified the 66-kDa subunit of splicing factor 3a (SF3a66) as a binding partner in a yeast two-hybrid screen and confirmed this interaction by pull-down assays. The splicing factor interacted with the 18-kDa (FGF-218) and with the 23-kDa (FGF-223) isoforms, indicating an interaction with a domain common to both isoforms. Moreover, FGF-2 interacted with the C-terminus of SF3a66, a sequence that has not previously been assigned a functional role. In a functional neurite outgrowth assay, SF3a66 enhanced neurite lengths similar to FGF-218. We have previously identified the spliceosomal assembly factor survival of motoneuron (SMN) protein as a protein interacting specifically with the FGF-223 isoform [Claus et al., J. Biol. Chem. 278 (2003), 479-485]. The identification of two FGF-2 interacting proteins from the same biochemical pathway suggests a novel intranuclear role of FGF-2. | |
| 540 | |a ©2004 by Walter de Gruyter Berlin New York | ||
| 690 | 7 | |a Biochemistry |2 nationallicence | |
| 690 | 7 | |a Molecular biology |2 nationallicence | |
| 690 | 7 | |a Cellular biology |2 nationallicence | |
| 690 | 7 | |a fibroblast growth factor 2 (FGF-2) |2 nationallicence | |
| 690 | 7 | |a growth factor signal transduction |2 nationallicence | |
| 690 | 7 | |a intracellular growth factor |2 nationallicence | |
| 690 | 7 | |a survival of motoneuron protein (SMN) |2 nationallicence | |
| 700 | 1 | |a Gringel |D Susanne |u Department of Neuroanatomy, Hannover Medical School, D-30625 Hannover, Germany |4 aut | |
| 700 | 1 | |a van Bergeijk |D Jeroen |u Department of Neuroanatomy, Hannover Medical School, D-30625 Hannover, Germany |4 aut | |
| 700 | 1 | |a Haastert |D Kirsten |u Department of Neuroanatomy, Hannover Medical School, D-30625 Hannover, Germany and Center for Systems Neuroscience (ZSN) Hannover, OE 4140, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany |4 aut | |
| 700 | 1 | |a Grothe |D Claudia |u Department of Neuroanatomy, Hannover Medical School, D-30625 Hannover, Germany and Center for Systems Neuroscience (ZSN) Hannover, OE 4140, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany |4 aut | |
| 700 | 1 | |a Claus |D Peter |u Department of Neuroanatomy, Hannover Medical School, D-30625 Hannover, Germany and Center for Systems Neuroscience (ZSN) Hannover, OE 4140, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany |4 aut | |
| 773 | 0 | |t Biological Chemistry |d Walter de Gruyter |g 385/12(2004-12-01), 1203-1208 |x 1431-6730 |q 385:12<1203 |1 2004 |2 385 |o bchm | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/BC.2004.156 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/BC.2004.156 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gringel |D Susanne |u Department of Neuroanatomy, Hannover Medical School, D-30625 Hannover, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a van Bergeijk |D Jeroen |u Department of Neuroanatomy, Hannover Medical School, D-30625 Hannover, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Haastert |D Kirsten |u Department of Neuroanatomy, Hannover Medical School, D-30625 Hannover, Germany and Center for Systems Neuroscience (ZSN) Hannover, OE 4140, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Grothe |D Claudia |u Department of Neuroanatomy, Hannover Medical School, D-30625 Hannover, Germany and Center for Systems Neuroscience (ZSN) Hannover, OE 4140, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Claus |D Peter |u Department of Neuroanatomy, Hannover Medical School, D-30625 Hannover, Germany and Center for Systems Neuroscience (ZSN) Hannover, OE 4140, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biological Chemistry |d Walter de Gruyter |g 385/12(2004-12-01), 1203-1208 |x 1431-6730 |q 385:12<1203 |1 2004 |2 385 |o bchm | ||
| 900 | 7 | |b CC0 |u http://creativecommons.org/publicdomain/zero/1.0 |2 nationallicence | |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-gruyter | ||