caa a22 4500 378896083 CHVBK 20180305123506.0 cr unu---uuuuu 161128e20041201xx s 000 0 eng 10.1515/BC.2004.156 doi (NATIONALLICENCE)gruyter-10.1515/BC.2004.156 Nuclear fibroblast growth factor-2 interacts specifically with splicing factor SF3a66 [Elektronische Daten] [Susanne Gringel, Jeroen van Bergeijk, Kirsten Haastert, Claudia Grothe, Peter Claus] Fibroblast growth factor 2 (FGF-2) has a dual role as a classical extracellular signaling protein and as an intracellular factor. Isoforms of FGF-2, resulting from alternatively used start codons on one mRNA species, locate differentially to nuclear compartments. In this study we aimed to analyze functions of intracellular FGF-2 by identification of interacting proteins. We identified the 66-kDa subunit of splicing factor 3a (SF3a66) as a binding partner in a yeast two-hybrid screen and confirmed this interaction by pull-down assays. The splicing factor interacted with the 18-kDa (FGF-218) and with the 23-kDa (FGF-223) isoforms, indicating an interaction with a domain common to both isoforms. Moreover, FGF-2 interacted with the C-terminus of SF3a66, a sequence that has not previously been assigned a functional role. In a functional neurite outgrowth assay, SF3a66 enhanced neurite lengths similar to FGF-218. We have previously identified the spliceosomal assembly factor survival of motoneuron (SMN) protein as a protein interacting specifically with the FGF-223 isoform [Claus et al., J. Biol. Chem. 278 (2003), 479-485]. The identification of two FGF-2 interacting proteins from the same biochemical pathway suggests a novel intranuclear role of FGF-2. ©2004 by Walter de Gruyter Berlin New York Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence fibroblast growth factor 2 (FGF-2) nationallicence growth factor signal transduction nationallicence intracellular growth factor nationallicence survival of motoneuron protein (SMN) nationallicence Gringel Susanne Department of Neuroanatomy, Hannover Medical School, D-30625 Hannover, Germany aut van Bergeijk Jeroen Department of Neuroanatomy, Hannover Medical School, D-30625 Hannover, Germany aut Haastert Kirsten Department of Neuroanatomy, Hannover Medical School, D-30625 Hannover, Germany and Center for Systems Neuroscience (ZSN) Hannover, OE 4140, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany aut Grothe Claudia Department of Neuroanatomy, Hannover Medical School, D-30625 Hannover, Germany and Center for Systems Neuroscience (ZSN) Hannover, OE 4140, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany aut Claus Peter Department of Neuroanatomy, Hannover Medical School, D-30625 Hannover, Germany and Center for Systems Neuroscience (ZSN) Hannover, OE 4140, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany aut Biological Chemistry Walter de Gruyter 385/12(2004-12-01), 1203-1208 1431-6730 385:12<1203 2004 385 bchm https://doi.org/10.1515/BC.2004.156 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2004.156 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gringel Susanne Department of Neuroanatomy, Hannover Medical School, D-30625 Hannover, Germany aut NATIONALLICENCE 700 1- van Bergeijk Jeroen Department of Neuroanatomy, Hannover Medical School, D-30625 Hannover, Germany aut NATIONALLICENCE 700 1- Haastert Kirsten Department of Neuroanatomy, Hannover Medical School, D-30625 Hannover, Germany and Center for Systems Neuroscience (ZSN) Hannover, OE 4140, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany aut NATIONALLICENCE 700 1- Grothe Claudia Department of Neuroanatomy, Hannover Medical School, D-30625 Hannover, Germany and Center for Systems Neuroscience (ZSN) Hannover, OE 4140, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany aut NATIONALLICENCE 700 1- Claus Peter Department of Neuroanatomy, Hannover Medical School, D-30625 Hannover, Germany and Center for Systems Neuroscience (ZSN) Hannover, OE 4140, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 385/12(2004-12-01), 1203-1208 1431-6730 385:12<1203 2004 385 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter