caa a22 4500 378897942 CHVBK 20180305123510.0 cr unu---uuuuu 161128e20040216xx s 000 0 eng 10.1515/CCLM.2004.008 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2004.008 Analytical performance and diagnostic accuracy of a fully-automated electrochemiluminescent assay for the N-terminal fragment of the pro-peptide of brain natriuretic peptide in patients with cardiomyopathy: comparison with immunoradiometric assay methods for brain natriuretic peptide and atrial natriuretic peptide [Elektronische Daten] [Concetta Prontera, Michele Emdin, Gian Carlo Zucchelli, Andrea Ripoli, Claudio Passino, Aldo Clerico] We evaluated the analytical performance of a fully-automated electrochemiluminescence "sandwich” immunoassay method for the N-terminal fragment of the pro-peptide of brain natriuretic peptide (BNP). We then compared the diagnostic accuracy of this method in discriminating between normal subjects and patients with cardiomyopathy with that found with two previously described immunoradiometric assay methods for the assay of atrial natriuretic peptide (ANP) and BNP. We studied 193 consecutive patients (mean age 64.4±12.3 years, range 20-89 years, including 56 women and 137 men) with chronic cardiomyopathy and a group of 85 healthy subjects (mean age 52.3±12.0 years, 42 women and 43 men, range 20-79 years). N-terminal fragment of proBNP1-76 (NT-proBNP) was measured with a fully-automated "sandwich” electrochemiluminescence method using an Elecsys® 2010 analyzer, while ANP and BNP were measured with immunoradiometric assay methods. The low detection limit of the NTproBNP assay was 4.2 pg/ml (0.50 pmol/l), while the functional sensitivity was 22 pg/ml (2.60 pmol/l) with a working range (imprecision profile ≤ 10% coefficient of variation) extended up to about 30 000 pg/ml (3540 pmol/l). Healthy women (64.3±41.6 pg/ml, 7.59±4.91 pmol/l) showed significantly higher values than men (46.9±30.9 pg/ml, 5.53±3.64 pmol/l, p=0.0118). Moreover, age and sex were significantly and independently related to the NT-proBNP values in healthy subjects, as assessed by a multiple linear regression analysis (R=0.389, F-value=7.316, P-value=0.0012). As expected, the NT-proBNP values of patients with cardiomyopathy were significantly higher than those of normal subjects and progressively increased with the severity of heart failure. The respective diagnostic accuracy of the ANP, BNP and NT-proBNP assays in discriminating between the group of normal subjects and that of patients with cardiomyopathy was tested by the response operating characteristic curve analysis. Our data indicated that the NT-proBNP assay is significantly better than either of the ANP or BNP immunoradiometric assays in discriminating affected patients from healthy subjects, especially when only patients with mild disease severity (New York Heart Association class I and II) are considered. Copyright (c) 2004 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Prontera Concetta aut Emdin Michele aut Carlo Zucchelli Gian aut Ripoli Andrea aut Passino Claudio aut Clerico Aldo aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/1(2004-02-16), 37-44 1434-6621 42:1<37 2004 42 cclm https://doi.org/10.1515/CCLM.2004.008 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2004.008 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Prontera Concetta aut NATIONALLICENCE 700 1- Emdin Michele aut NATIONALLICENCE 700 1- Carlo Zucchelli Gian aut NATIONALLICENCE 700 1- Ripoli Andrea aut NATIONALLICENCE 700 1- Passino Claudio aut NATIONALLICENCE 700 1- Clerico Aldo aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/1(2004-02-16), 37-44 1434-6621 42:1<37 2004 42 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter