Isolation of plasma small-dense low-density lipoprotein using a simple air-driven ultracentrifuge and quantification using immunassay of apolipoprotein B
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| 024 | 7 | 0 | |a 10.1515/CCLM.2004.007 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/CCLM.2004.007 | ||
| 245 | 0 | 0 | |a Isolation of plasma small-dense low-density lipoprotein using a simple air-driven ultracentrifuge and quantification using immunassay of apolipoprotein B |h [Elektronische Daten] |c [Valentine C. Menys, Yifen Liu, Michael I. Mackness, See Kwok, Muriel J. Caslake, Grace Stewart, Paul N. Durrington] |
| 520 | 3 | |a Small-dense low-density lipoprotein (SD-LDL) is associated with coronary heart disease risk. Current methods for its quantification are expensive, complex and time-consuming. Plasma was adjusted to a density (D) of 1.044 g/ml in a volume of 0.18 ml and centrifuged in a Beckman Airfuge at 160 000×g for 3 h 7 min and apolipoprotein B (apoB) then determined in the infranatant. Results were compared with centrifugation of 5 ml of plasma at D=1.044 g/ml at 144 000×g for 18 h in a preparative ultracentrifuge (UC). We obtained blood samples from healthy subjects (n=73) and from dyslipidaemic patients (n=112). SD-LDL apoB levels as determined using the UC method ranged from 0.01-0.43 g/l and there was a good correlation with Airfuge results (r=0.925; p<0.0001; n=185). There was a mean difference of 0.0125 g/l between methods. SD-LDL apoB levels as determined using the Airfuge showed a reasonable agreement with results obtained using density gradient ultracentrifugation (r=0.773; p<0.01; n=12). Airfuge results correlated directly with triglyceride concentration, in both healthy men (r=0.296; p<0.05) and dyslipidaemic men (r=0.520; p<0.001) and also in dyslipidaemic women (r=0.463; p<0.005). Airfuge results correlated inversely with high-density lipoprotein-cholesterol (HDL-C) concentration, in both healthy men (r=−0.237; p<0.05) and dyslipidaemic men (r=−0.293; p<0.005). Using a micro-method, we obtained results which establish that the Airfuge provides a more rapid and less expensive method for quantification of SD-LDL. | |
| 540 | |a Copyright © 2004 by Walter de Gruyter GmbH & Co. KG | ||
| 690 | 7 | |a Medical equipment & techniques |2 nationallicence | |
| 690 | 7 | |a Medical diagnosis |2 nationallicence | |
| 690 | 7 | |a Diseases & disorders |2 nationallicence | |
| 700 | 1 | |a Menys |D Valentine C. |4 aut | |
| 700 | 1 | |a Liu |D Yifen |4 aut | |
| 700 | 1 | |a Mackness |D Michael I. |4 aut | |
| 700 | 1 | |a Kwok |D See |4 aut | |
| 700 | 1 | |a Caslake |D Muriel J. |4 aut | |
| 700 | 1 | |a Stewart |D Grace |4 aut | |
| 700 | 1 | |a Durrington |D Paul N. |4 aut | |
| 773 | 0 | |t Clinical Chemistry and Laboratory Medicine |d Walter de Gruyter |g 42/1(2004-02-16), 30-36 |x 1434-6621 |q 42:1<30 |1 2004 |2 42 |o cclm | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/CCLM.2004.007 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/CCLM.2004.007 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Menys |D Valentine C. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Yifen |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mackness |D Michael I. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kwok |D See |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Caslake |D Muriel J. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Stewart |D Grace |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Durrington |D Paul N. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Clinical Chemistry and Laboratory Medicine |d Walter de Gruyter |g 42/1(2004-02-16), 30-36 |x 1434-6621 |q 42:1<30 |1 2004 |2 42 |o cclm | ||
| 900 | 7 | |b CC0 |u http://creativecommons.org/publicdomain/zero/1.0 |2 nationallicence | |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-gruyter | ||