caa a22 4500 378897969 CHVBK 20180305123510.0 cr unu---uuuuu 161128e20040216xx s 000 0 eng 10.1515/CCLM.2004.007 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2004.007 Isolation of plasma small-dense low-density lipoprotein using a simple air-driven ultracentrifuge and quantification using immunassay of apolipoprotein B [Elektronische Daten] [Valentine C. Menys, Yifen Liu, Michael I. Mackness, See Kwok, Muriel J. Caslake, Grace Stewart, Paul N. Durrington] Small-dense low-density lipoprotein (SD-LDL) is associated with coronary heart disease risk. Current methods for its quantification are expensive, complex and time-consuming. Plasma was adjusted to a density (D) of 1.044 g/ml in a volume of 0.18 ml and centrifuged in a Beckman Airfuge at 160 000×g for 3 h 7 min and apolipoprotein B (apoB) then determined in the infranatant. Results were compared with centrifugation of 5 ml of plasma at D=1.044 g/ml at 144 000×g for 18 h in a preparative ultracentrifuge (UC). We obtained blood samples from healthy subjects (n=73) and from dyslipidaemic patients (n=112). SD-LDL apoB levels as determined using the UC method ranged from 0.01-0.43 g/l and there was a good correlation with Airfuge results (r=0.925; p<0.0001; n=185). There was a mean difference of 0.0125 g/l between methods. SD-LDL apoB levels as determined using the Airfuge showed a reasonable agreement with results obtained using density gradient ultracentrifugation (r=0.773; p<0.01; n=12). Airfuge results correlated directly with triglyceride concentration, in both healthy men (r=0.296; p<0.05) and dyslipidaemic men (r=0.520; p<0.001) and also in dyslipidaemic women (r=0.463; p<0.005). Airfuge results correlated inversely with high-density lipoprotein-cholesterol (HDL-C) concentration, in both healthy men (r=−0.237; p<0.05) and dyslipidaemic men (r=−0.293; p<0.005). Using a micro-method, we obtained results which establish that the Airfuge provides a more rapid and less expensive method for quantification of SD-LDL. Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Menys Valentine C. aut Liu Yifen aut Mackness Michael I. aut Kwok See aut Caslake Muriel J. aut Stewart Grace aut Durrington Paul N. aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/1(2004-02-16), 30-36 1434-6621 42:1<30 2004 42 cclm https://doi.org/10.1515/CCLM.2004.007 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2004.007 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Menys Valentine C. aut NATIONALLICENCE 700 1- Liu Yifen aut NATIONALLICENCE 700 1- Mackness Michael I. aut NATIONALLICENCE 700 1- Kwok See aut NATIONALLICENCE 700 1- Caslake Muriel J. aut NATIONALLICENCE 700 1- Stewart Grace aut NATIONALLICENCE 700 1- Durrington Paul N. aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/1(2004-02-16), 30-36 1434-6621 42:1<30 2004 42 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter