caa a22 4500 378898051 CHVBK 20180305123511.0 cr unu---uuuuu 161128e20040216xx s 000 0 eng 10.1515/CCLM.2004.006 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2004.006 Limited usefulness of the PFA-100™ for the monitoring of ADP receptor antagonists-in vitro experience [Elektronische Daten] [Jacek Golanski, Justyna Pluta, Janina Baraniak, Cezary Watala] We have evaluated the usefulness of the PFA-100™ system (collagen/ADP and collagen/epinephrine cartridges) to assess the in vitro effects of a few platelet function inhibitors: Aspisol® (60 μg/ml), 4-[4-[4-(aminoiminomethyl]-1-piperazinyl]-1-piperidineactetic acid, hydrochloride trihydrate (GR144053F, fibrinogen receptor antagonist, 100 nM), adenosine-3′,5′-diphosphate (A3P5P, P2Y1 ADP receptor antagonist, 500 μM) and Bis[(adenosine-5′-O-phosphorodithioyl) methylene]-phosphinic acid (APTMPA, P2Y12 ADP receptor antagonist, 500 μM) on platelet function, as compared with the other commonly used diagnostic technique, a whole blood electrical aggregometry (20 μM ADP-or 0.5 mM arachidonic acid). The in vitro studies were carried out on a group of 38 subjects. Whereas all the examined platelet antagonists and inhibitors almost completely blocked the 20 mM ADP-or 0.5 mM arachidonic acid-induced (in the case of acetylsalicylic acid) whole blood aggregation, only two inhibitors (Aspisol® and GR144053F) remained effective in a significant prolongation of the PFA-100™ occlusion time. Otherwise, using the PFA-100™ system we were not able to detect the inhibitory actions of ADP receptor antagonists-P2Y1 and P2Y12. Our findings point to a limited usefulness of the PFA-100™ system for the monitoring of the effectiveness of ADP receptor antagonists. The outcomes of this study show that platelet aggregometry in whole blood is characterised by the highest sensitivity in the monitoring of the investigated blood platelet inhibitors. Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Golanski Jacek aut Pluta Justyna aut Baraniak Janina aut Watala Cezary aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/1(2004-02-16), 25-29 1434-6621 42:1<25 2004 42 cclm https://doi.org/10.1515/CCLM.2004.006 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2004.006 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Golanski Jacek aut NATIONALLICENCE 700 1- Pluta Justyna aut NATIONALLICENCE 700 1- Baraniak Janina aut NATIONALLICENCE 700 1- Watala Cezary aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/1(2004-02-16), 25-29 1434-6621 42:1<25 2004 42 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter