caa a22 4500 378898280 CHVBK 20180305123511.0 cr unu---uuuuu 161128e20041201xx s 000 0 eng 10.1515/CCLM.2004.260 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2004.260 Analysis of inflammatory response in human plasma samples by an automated multicapillary electrophoresis system [Elektronische Daten] [Anders Larsson, Lars-Olof Hansson] A new automated multicapillary zone electrophoresis instrument with a new high-resolution (HR) buffer (Capillarys™ with HR buffer) for analysis of human plasma proteins was evaluated. Albumin, α1-antitrypsin, α1-acid glycoprotein, haptoglobin, fibrinogen, immunoglobulin (Ig)A, IgG and IgM were determined nephelometrically in 200 patient plasma samples. The same samples were then analyzed on the Capillarys™ system (Sebia, Paris, France). The albumin concentration from the nephelometric determination was used for quantification of the individual peaks in the capillary electrophoresis (CE) electropherogram. There was strong linear correlation between the nephelometric and electrophoretic determination of α1-antitrypsin (R2=0.906), α1-acid glycoprotein (R2=0.894) and haptoglobin (R2=0.913). There was also good correlation between the two determinations of γ-globulins (R2=0.883), while the correlation was weaker for fibrinogen (R2=0.377). The Capillarys™ instrument is a reliable system for plasma protein analysis, combining the advantages of full automation, good analytical performance and high throughput. The HR buffer in combination with albumin quantification allows the simultaneous quantification of inflammatory markers in plasma samples without the need for nephelometric determination of these proteins. ©2004 by Walter de Gruyter Berlin New York Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence agarose gel electrophoresis nationallicence capillary electrophoresis nationallicence human nationallicence inflammatory response nationallicence nephelometry nationallicence plasma nationallicence Larsson Anders Department of Medical Sciences, Clinical Chemistry, University Hospital, Uppsala, Sweden aut Hansson Lars-Olof Department of Medical Sciences, Clinical Chemistry, University Hospital, Uppsala, Sweden aut Clinical Chemical Laboratory Medicine Walter de Gruyter 42/12(2004-12-01), 1396-1400 1434-6621 42:12<1396 2004 42 cclm https://doi.org/10.1515/CCLM.2004.260 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2004.260 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Larsson Anders Department of Medical Sciences, Clinical Chemistry, University Hospital, Uppsala, Sweden aut NATIONALLICENCE 700 1- Hansson Lars-Olof Department of Medical Sciences, Clinical Chemistry, University Hospital, Uppsala, Sweden aut NATIONALLICENCE 773 0- Clinical Chemical Laboratory Medicine Walter de Gruyter 42/12(2004-12-01), 1396-1400 1434-6621 42:12<1396 2004 42 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter