caa a22 4500 378901818 CHVBK 20180305123519.0 cr unu---uuuuu 161128e20040701xx s 000 0 eng 10.2298/hel0440099v doi (NATIONALLICENCE)gruyter-10.2298/hel0440099v DEVELOPMENT OF SUNFLOWER GERMPLASM WITH HIGH DELTA-TOCOPHEROL CONTENT [Elektronische Daten] FORMACIÓN DE GERMOPLASMA DE GIRASOL CON ALTO CONTENIDO DE TOCOFEROL DELTA / FORMATION DE GERMES PLASMIQUES DE TOURNESOL D'UN CONTENU ÉLEVÉ DE DELTA-TOCOPHÉROL / [L. Velasco, B. Pérez-Vich, J.M. Fernández-Martínez] Tocopherols are the main compounds with antioxidant activity in oilseeds. Sunflower seeds contain predominantly alpha-tocopherol, which accounts for more than 90% of the total tocopherols in the seeds. This tocopherol derivative possesses a maximum vitamin E or in vivo antioxidant activity, but it exerts a minimum in vitro protective action in oils and food containing them. Other tocopherol derivatives such as beta-, gamma-, and delta-tocopherol are more powerful antioxidants than alpha-tocopherol. Accordingly, a partial replacement of alpha-tocopherol in sunflower seeds is an important breeding objective. So far, variants with high gamma tocopherol content (>85%), medium beta-tocopherol content (30% to 50%), and medium delta-tocopherol content (<25%) have been developed. The objective of the present research was to develop sunflower germplasm with novel tocopherol profiles. Seed of four Peredovik accessions of different origins were used for chemical mutagenesis with ethyl methane sulfonate (EMS). Single-seed screening in the M2 generation resulted in the identification of an M2 seed with 19% gamma-tocopherol. M3 seeds exhibited wide segregation for gamma-tocopherol content, from zero to about 85%. Such a wide segregation has not been observed in previously developed high gamma-tocopherol germplasms, which segregate for low and high but not for intermediate levels of gamma-tocopherol. Selection for high gamma-tocopherol content within this mutant produced an M4:5 line with stable high concentration of gamma-tocopherol, above 90% of the total tocopherols. The line was designated IAST-1. Crosses between IAST-1 and the line T589, with medium beta-tocopherol content, produced segregants with increased levels of up to 68% delta-tocopherol. This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. antioxidants nationallicence high delta-tocopherol content nationallicence mutagenesis nationallicence sunflower seeds nationallicence tocopherols nationallicence Velasco L. Instituto de Agricultura Sostenible (CSIC), Alameda del Obispo s/n, E-14004 Córdoba, Spain aut Pérez-Vich B. Instituto de Agricultura Sostenible (CSIC), Alameda del Obispo s/n, E-14004 Córdoba, Spain aut Fernández-Martínez J.M. Instituto de Agricultura Sostenible (CSIC), Alameda del Obispo s/n, E-14004 Córdoba, Spain, Phone: +34-957499204, Fax: +34-957499252 aut HELIA De Gruyter 27/40(2004-07-01), 99-106 1018-1806 27:40<99 2004 27 helia https://doi.org/10.2298/hel0440099v text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.2298/hel0440099v text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Velasco L. Instituto de Agricultura Sostenible (CSIC), Alameda del Obispo s/n, E-14004 Córdoba, Spain aut NATIONALLICENCE 700 1- Pérez-Vich B. Instituto de Agricultura Sostenible (CSIC), Alameda del Obispo s/n, E-14004 Córdoba, Spain aut NATIONALLICENCE 700 1- Fernández-Martínez J.M. Instituto de Agricultura Sostenible (CSIC), Alameda del Obispo s/n, E-14004 Córdoba, Spain, Phone: +34-957499204, Fax: +34-957499252 aut NATIONALLICENCE 773 0- HELIA De Gruyter 27/40(2004-07-01), 99-106 1018-1806 27:40<99 2004 27 helia CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter