caa a22 4500 37890194X CHVBK 20180305123520.0 cr unu---uuuuu 161128e20040701xx s 000 0 eng 10.2298/hel0440063t doi (NATIONALLICENCE)gruyter-10.2298/hel0440063t HALF-SEED ANALYSIS FOR COMPARING LINOLENIC ACID SYNTHESIS BETWEEN HIGH AND LOW OLEIC ACID SUNFLOWER INBRED LINES [Elektronische Daten] ANÁLISIS REALIZADO UTILIZANDO LA MITAD DE LA SEMILLA PARA LA COMPARACIÓN DE LA SÍNTESIS DEL ÁCIDO LINOLÉNICO EN LAS LÍNEAS CONSANGUÍNEAS DE GIRASOL CON ALTO Y BAJO CONTENIDO DE ÁCIDO OLEICO / ANALYSE DE GRAINES À DEMI POUR COMPARER LES SYNTHÈSES ENTRE LES ACIDES OLÉIQUES ET LINOLÉIQUES DANS LES LIGNES CULTIVÉES DE TOURNESOL / [S. Tahmasebi-Enferadi, Z. Rabiei, M. Turi, M. Baldini, G.P. Vannozzi] The modification of fatty acid composition of two sunflowers inbred lines, HA89 and R978, low oleic acid (normal) and high oleic acid (mutant) respectively, in seeds and during the first stages of growth (A1-B2) was studied under controlled conditions. Enzymatic mechanisms have great effect on the catabolism of seed stored lipids. Temperature and oxygen regulation influence developing sunflower seeds. For simultaneous study of seed and developing seed, half-seed analysis technique was used. The behavior of the fatty acids during the germination in cotyledon of seed showed the increase of linolenic acid in both lines, demonstrating the activity of linoleic acid desaturase (Δ6-desaturase). But linoleic acid as a substrate for linoleate desaturase increased during all stages of developing only in mutant line that revealed higher activity of oleic acid desaturase (Δ12-desaturase) in transforming oleic acid to linoleic acid in this line, and lower activity of this enzyme in low oleic acid line, the reasons probably being the low availability of substrate of this enzyme in low oleic acid line and the complexity of enzymatic mechanisms. The modification of fatty acids in developing sunflower, depends not only on ambient conditions such as temperature and oxygen regulation as described by many authors, but also on the genotype. This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. half-seed analysis nationallicence linolenic acid nationallicence linoleic acid desaturase nationallicence oleic acid desaturase nationallicence sunflower nationallicence Tahmasebi-Enferadi S. Dipartimento Produzione Vegetale e Tecnologie Agrarie,Università di Udine, via delle Scienze 208, 33100 Udine Italy, Phone: +39(0432) 558665, Fax: +39 (0432) 558603 aut Rabiei Z. Dipartimento Produzione Vegetale e Tecnologie Agrarie,Università di Udine, via delle Scienze 208, 33100 Udine Italy aut Turi M. Dipartimento Produzione Vegetale e Tecnologie Agrarie,Università di Udine, via delle Scienze 208, 33100 Udine Italy aut Baldini M. Dipartimento Produzione Vegetale e Tecnologie Agrarie,Università di Udine, via delle Scienze 208, 33100 Udine Italy aut Vannozzi G.P. Dipartimento Produzione Vegetale e Tecnologie Agrarie,Università di Udine, via delle Scienze 208, 33100 Udine Italy aut HELIA De Gruyter 27/40(2004-07-01), 63-72 1018-1806 27:40<63 2004 27 helia https://doi.org/10.2298/hel0440063t text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.2298/hel0440063t text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tahmasebi-Enferadi S. Dipartimento Produzione Vegetale e Tecnologie Agrarie,Università di Udine, via delle Scienze 208, 33100 Udine Italy, Phone: +39(0432) 558665, Fax: +39 (0432) 558603 aut NATIONALLICENCE 700 1- Rabiei Z. Dipartimento Produzione Vegetale e Tecnologie Agrarie,Università di Udine, via delle Scienze 208, 33100 Udine Italy aut NATIONALLICENCE 700 1- Turi M. Dipartimento Produzione Vegetale e Tecnologie Agrarie,Università di Udine, via delle Scienze 208, 33100 Udine Italy aut NATIONALLICENCE 700 1- Baldini M. Dipartimento Produzione Vegetale e Tecnologie Agrarie,Università di Udine, via delle Scienze 208, 33100 Udine Italy aut NATIONALLICENCE 700 1- Vannozzi G.P. Dipartimento Produzione Vegetale e Tecnologie Agrarie,Università di Udine, via delle Scienze 208, 33100 Udine Italy aut NATIONALLICENCE 773 0- HELIA De Gruyter 27/40(2004-07-01), 63-72 1018-1806 27:40<63 2004 27 helia CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter