caa a22 4500 378901982 CHVBK 20180305123520.0 cr unu---uuuuu 161128e20040701xx s 000 0 eng 10.2298/hel0440015k doi (NATIONALLICENCE)gruyter-10.2298/hel0440015k CONSTRUCTION OF A GENETIC MAP AND LOCALIZATION OF MAJOR TRAITS IN SUNFLOWER (Helianthus annuus L.) [Elektronische Daten] CONSTRUCCIÓN DEL MAPA GENÉTICO Y UBICACIÓN DE LAS CARACTERÍSTICAS MÁS IMPORTANTES EN GIRASOL (Helianthus annuus L.) / CONSTRUCTION DE CARTES GÉNÉTIQUES ET LOCALISATION DE TRAITS PERTINENTS DU TOURNESOL (Helianthus annuus L.) / [B. Kusterer, B. Rozynek, L. Brahm, M. Prüfe, S. Tzigos, R. Horn, W. Friedt] We have developed a segregating mapping population from the cross RHA325(cms) × HA342 comprising 183 F2 individuals. RHA325 is an open American inbred line based on the PET1 cytoplasm while HA342 is a maintainer line for this cytoplasm. The F2 and derived populations segregate for male fertility vs. sterility, downy mildew (Plasmopara halstedii) resistance (Pl2) vs. susceptibility and oleic vs. linoleic acid content. A genetic map was developed covering 1751.5 cM with 202 AFLP and 19 SSR markers in 18 linkage groups. Thirteen linkage groups contain one or more SSR markers and are numbered according to Tang et al. (2002). A segregation ratio of 1 (male fertile, Rf1Rf1) : 2 (male fertile, Rf1rf1) : 1 (male sterile, rf1rf1) was observed in the F2 population as expected for one segregating restorer gene (χ2=2.83, P=0.24). In F3, 14 progeny plants of each fertile F2 individual were evaluated for male fertility to distinguish between F2 plants being homozygous or heterozygous for the restorer gene. The data obtained from the F3 progenies were confirmed by segregation analyses in an F2BC1 population. The Rf1 locus was shown to be located on linkage group 13, containing the SSR markers ORS388 and ORS1030. Using a whole-seedling-immersion test (Gulya et al., 1991) applied to F3 progeny plants we found that the F2 population RHA325(cms) × HA342 segregated for Plasmopara reaction at a ratio of 1 (Pl2Pl2) :2 (Pl2pl2) :1 (pl2pl2) ;χ2=0.83 (P=0.65). The Pl2 gene was demonstrated to be located on linkage group 8 together with the SSR marker ORS599. Furthermore, quantitative trait loci for oleic vs. linoleic acid contents (LOD >3) could be localized on linkage group A. Future work will concentrate on marker saturation of the genetic map. This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. cytoplasmic male sterility nationallicence genomics nationallicence hybrid breeding nationallicence molecular markers nationallicence mapping nationallicence Plasmopara halstedii nationallicence oleic acid nationallicence QTL nationallicence Kusterer B. Institute of Crop Science and Plant Breeding I, IFZ, Justus-Liebig-University Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany aut Rozynek B. Institute of Crop Science and Plant Breeding I, IFZ, Justus-Liebig-University Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany aut Brahm L. Institute of Crop Science and Plant Breeding I, IFZ, Justus-Liebig-University Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany aut Prüfe M. Institute of Crop Science and Plant Breeding I, IFZ, Justus-Liebig-University Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany aut Tzigos S. Institute of Crop Science and Plant Breeding I, IFZ, Justus-Liebig-University Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany aut Horn R. Institute of Crop Science and Plant Breeding I, IFZ, Justus-Liebig-University Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany aut Friedt W. Institute of Crop Science and Plant Breeding I, IFZ, Justus-Liebig-University Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany aut HELIA De Gruyter 27/40(2004-07-01), 15-24 1018-1806 27:40<15 2004 27 helia https://doi.org/10.2298/hel0440015k text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.2298/hel0440015k text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kusterer B. Institute of Crop Science and Plant Breeding I, IFZ, Justus-Liebig-University Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany aut NATIONALLICENCE 700 1- Rozynek B. Institute of Crop Science and Plant Breeding I, IFZ, Justus-Liebig-University Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany aut NATIONALLICENCE 700 1- Brahm L. Institute of Crop Science and Plant Breeding I, IFZ, Justus-Liebig-University Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany aut NATIONALLICENCE 700 1- Prüfe M. Institute of Crop Science and Plant Breeding I, IFZ, Justus-Liebig-University Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany aut NATIONALLICENCE 700 1- Tzigos S. Institute of Crop Science and Plant Breeding I, IFZ, Justus-Liebig-University Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany aut NATIONALLICENCE 700 1- Horn R. Institute of Crop Science and Plant Breeding I, IFZ, Justus-Liebig-University Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany aut NATIONALLICENCE 700 1- Friedt W. Institute of Crop Science and Plant Breeding I, IFZ, Justus-Liebig-University Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany aut NATIONALLICENCE 773 0- HELIA De Gruyter 27/40(2004-07-01), 15-24 1018-1806 27:40<15 2004 27 helia CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter