caa a22 4500 378902008 CHVBK 20180305123520.0 cr unu---uuuuu 161128e20040701xx s 000 0 eng 10.2298/hel0440107v doi (NATIONALLICENCE)gruyter-10.2298/hel0440107v EVALUATION OF WILD SUNFLOWER SPECIES FOR TOCOPHEROL CONTENT AND COMPOSITION [Elektronische Daten] EVALUACIÓN DEL CONTENIDO Y COMPOSICIÓN DE TOCOFEROL EN LAS ESPECIES SILVESTRES DE GIRASOL / EVALUATION DE CONTENU ET COMPOSITION DE TOCOPHÉROL CHEZ LES ESPECES SAUVAGES DE TOURNESOL / [L. Velasco, B. Pérez-Vich, J.M. Fernández-Martínez] Wild Helianthus species are an important reservoir of useful genes for sunflower breeding. The objective of the present research was to evaluate the existing variability for tocopherol content and composition in a germplasm collection of wild sunflower species. The germplasm evaluated consisted of 257 accessions from 36 Helianthus species, including both annual and perennial species. Eight seeds per accession were randomly picked and bulked for tocopherol analysis conducted by HPLC with fluorescence detection. Tocopherol content averaged 328 mg kg-1 seed in the analyzed accessions, with an average profile of 99.0% alpha-tocopherol, 0.7% beta-tocopherol, and 0.3% gammatocopherol. In cultivated material, an average tocopherol content of 669.1 mg kg-1 seed have been reported, made up of 92.4% alpha-tocopherol, 5.6% betatocopherol, and 2.0% gamma-tocopherol. The maximum total tocopherol content in wild sunflower germplasm corresponded to an accession of H. maximilianii, with 673 mg kg-1 seed. Increased levels of beta-tocopherol were identified in one accession of H. praecox (11.2% of the total tocopherols) and one accession of H. debilis (11.8%). All other accessions contained less than 6.5% betatocopherol. Increased gamma-tocopherol levels were identified in one accession of H. exilis (7.4%) and two accessions of H. nutalii (11.0% and 14.6%, respectively). All other accessions contained less than 2% gamma-tocopherol. Although further research at an intrapopulation level is needed to confirm and isolate variants, the results of the present research indicated that wild Helianthus germplasm contains useful variability for tocopherol content and composition. This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. genetic diversity nationallicence germplasm evaluation nationallicence Helianthus spp nationallicence tocopherols nationallicence wild species nationallicence variability nationallicence Velasco L. Instituto de Agricultura Sostenible (CSIC), Alameda del Obispo s/n, E-14004 Córdoba, Spain, Phone: +34-957499236; Fax: +34-957499252 aut Pérez-Vich B. Instituto de Agricultura Sostenible (CSIC), Alameda del Obispo s/n, E-14004 Córdoba, Spain aut Fernández-Martínez J.M. Instituto de Agricultura Sostenible (CSIC), Alameda del Obispo s/n, E-14004 Córdoba, Spain aut HELIA De Gruyter 27/40(2004-07-01), 107-112 1018-1806 27:40<107 2004 27 helia https://doi.org/10.2298/hel0440107v text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.2298/hel0440107v text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Velasco L. Instituto de Agricultura Sostenible (CSIC), Alameda del Obispo s/n, E-14004 Córdoba, Spain, Phone: +34-957499236; Fax: +34-957499252 aut NATIONALLICENCE 700 1- Pérez-Vich B. Instituto de Agricultura Sostenible (CSIC), Alameda del Obispo s/n, E-14004 Córdoba, Spain aut NATIONALLICENCE 700 1- Fernández-Martínez J.M. Instituto de Agricultura Sostenible (CSIC), Alameda del Obispo s/n, E-14004 Córdoba, Spain aut NATIONALLICENCE 773 0- HELIA De Gruyter 27/40(2004-07-01), 107-112 1018-1806 27:40<107 2004 27 helia CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter