caa a22 4500 378902032 CHVBK 20180305123520.0 cr unu---uuuuu 161128e20040701xx s 000 0 eng 10.2298/hel0440149p doi (NATIONALLICENCE)gruyter-10.2298/hel0440149p THE USE OF PCR-BASED MARKERS IN THE EVALUATION OF RESISTANCE TO DOWNY MILDEW IN NS-BREEDING MATERIAL [Elektronische Daten] USO DE LOS MARCADORES PCR EN LA INVESTIGACIÓN DE RESISTENCIA A TIZÓN DE GENOTIPOS DEL PROGRAMA DE MEJORAMIENTO DE NS / USAGE DES MARQUEURS PCR DANS L'ÉVALUATION DE RÉSISTANCE DE GÉNOTYPES À LA ROUILLE D'APRÈS LE PROGRAMME DE PLANTES CULTIVÉES DE NOVI SAD / [D. Panković, S. Jocić, N. Lačok, Z. Sakač, D. Škorić] The aim of this work was the application of previously developed and development of new PCR markers for the evaluation of sunflower resistance to downy mildew. Twenty sunflower inbred lines were investigated. Plant resistance to downy mildew was determined by the whole seedling immersion method. Genomic DNA was extracted from the first pair of leaves, and its polymorphism was investigated by RAPD, SSR and several published markers for disease resistance. The presence of the markers Ha-NBS 7/R, Ha-NBS 8/R, Ha-NBS 9/R, in Pl6 donor lines (Ha-335, JM-8) and in resistant progeny (Ha-26, G12, G10, G11) confirm that HAP3 could be useful for the detection of the Pl6 gene. DNA polymorphism, which coincided with disease resistance, was revealed with one RAPD (UBC 119 fragment 900-1000 bp) and one SSR primer (ORS37 fragment 600-700 bp). Amplified fragments segregated in the same way, i.e., they appeared in 50% of the resistant genotypes. The non-expecting SSR fragment was purified, cloned and sequenced. The results indicated that this fragment is not a part of a coding sequence. Specific primers for the amplification of this fragment have been designed and the investigation of the inheritance of this SCAR marker is under way. None of the applied markers appeared in all resistant genotypes. In order to select lines for making crosses for use in further investigation, the obtained results were also used for the calculation of genetic distances between genotypes (simple matching coefficient) and the construction of a dendrogram (UPGMA method). This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. sunflower nationallicence downy mildew nationallicence RAPD nationallicence SSR nationallicence DNA polymorphism nationallicence cluster analysis nationallicence Panković D. Institute of Field and Vegetable Crops, Maksima Gorkog 30., 21000 Novi Sad, Serbia and Montenegro, Phone: ++381 21 4898 416, Fax: ++381 21 413 833 aut Jocić S. Institute of Field and Vegetable Crops, Maksima Gorkog 30., 21000 Novi Sad, Serbia and Montenegro aut Lačok N. Institute of Field and Vegetable Crops, Maksima Gorkog 30., 21000 Novi Sad, Serbia and Montenegro aut Sakač Z. Institute of Field and Vegetable Crops, Maksima Gorkog 30., 21000 Novi Sad, Serbia and Montenegro aut Škorić D. Institute of Field and Vegetable Crops, Maksima Gorkog 30., 21000 Novi Sad, Serbia and Montenegro aut HELIA De Gruyter 27/40(2004-07-01), 149-158 1018-1806 27:40<149 2004 27 helia https://doi.org/10.2298/hel0440149p text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.2298/hel0440149p text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Panković D. Institute of Field and Vegetable Crops, Maksima Gorkog 30., 21000 Novi Sad, Serbia and Montenegro, Phone: ++381 21 4898 416, Fax: ++381 21 413 833 aut NATIONALLICENCE 700 1- Jocić S. Institute of Field and Vegetable Crops, Maksima Gorkog 30., 21000 Novi Sad, Serbia and Montenegro aut NATIONALLICENCE 700 1- Lačok N. Institute of Field and Vegetable Crops, Maksima Gorkog 30., 21000 Novi Sad, Serbia and Montenegro aut NATIONALLICENCE 700 1- Sakač Z. Institute of Field and Vegetable Crops, Maksima Gorkog 30., 21000 Novi Sad, Serbia and Montenegro aut NATIONALLICENCE 700 1- Škorić D. Institute of Field and Vegetable Crops, Maksima Gorkog 30., 21000 Novi Sad, Serbia and Montenegro aut NATIONALLICENCE 773 0- HELIA De Gruyter 27/40(2004-07-01), 149-158 1018-1806 27:40<149 2004 27 helia CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter