caa a22 4500 378904671 CHVBK 20180305123527.0 cr unu---uuuuu 161128e20040901xx s 000 0 eng 10.1515/CCLM.2004.210 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2004.210 Comparison between creatinine and pregnanediol adjustments in the retrospective analysis of urinary hormone profiles during the human menstrual cycle [Elektronische Daten] [Fernando Miro, John Coley, Mohamed M. Gani, Paul W. Perry, Duncan Talbot, Laurence J. Aspinall] Measurement of reproductive hormones in urine is a practical way of obtaining large amounts of information; however, there is still controversy on how to overcome problems derived from volume fluctuations between samples. Creatinine adjustment is a widely accepted solution, however, it introduces an extra cost, and large studies involving multiple sequential determinations would benefit from more economical solutions. We determined the value of creatinine adjustment, and compared it with a mathematical method that uses the smoothed profile of pregnanediol (PdG) as a reference to adjust other hormonal markers. To do this, we investigated the effects on three major urinary reproductive hormonal markers (luteinizing hormone (LH), estrone 3-glucuronide (E1G) and PdG) in 17 complete menstrual cycles. Detection of the day of LH peak did not differ between raw and adjusted data. Creatinine adjustment reduced variation in pre-ovulatory E1G levels between individuals, though the effect was negligible within individuals. No significant differences were found regarding post-ovulatory PdG rise. Although creatinine adjustment significantly reduces variability, producing smoother profiles, an equivalent degree of smoothness is obtained using the PdG adjustment. We conclude that under the current technology, for the retrospective study of urinary hormonal profiles in the human menstrual cycle, PdG adjustment is a valid alternative to creatinine. © Walter de Gruyter Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence creatinine nationallicence luteinizing hormone nationallicence menstrual cycle nationallicence pregnanediol nationallicence steroids nationallicence Miro Fernando Unipath Limited, Stannard Way, Priory Business Park, Bedford, United Kingdom aut Coley John Unipath Limited, Stannard Way, Priory Business Park, Bedford, United Kingdom aut Gani Mohamed M. Unipath Limited, Stannard Way, Priory Business Park, Bedford, United Kingdom aut Perry Paul W. Unipath Limited, Stannard Way, Priory Business Park, Bedford, United Kingdom aut Talbot Duncan Unilever Research Colworth, Sharnbrook, Bedford, United Kingdom aut Aspinall Laurence J. Unilever Research Colworth, Sharnbrook, Bedford, United Kingdom aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/9(2004-09-01), 1043-1050 1434-6621 42:9<1043 2004 42 cclm https://doi.org/10.1515/CCLM.2004.210 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2004.210 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miro Fernando Unipath Limited, Stannard Way, Priory Business Park, Bedford, United Kingdom aut NATIONALLICENCE 700 1- Coley John Unipath Limited, Stannard Way, Priory Business Park, Bedford, United Kingdom aut NATIONALLICENCE 700 1- Gani Mohamed M. Unipath Limited, Stannard Way, Priory Business Park, Bedford, United Kingdom aut NATIONALLICENCE 700 1- Perry Paul W. Unipath Limited, Stannard Way, Priory Business Park, Bedford, United Kingdom aut NATIONALLICENCE 700 1- Talbot Duncan Unilever Research Colworth, Sharnbrook, Bedford, United Kingdom aut NATIONALLICENCE 700 1- Aspinall Laurence J. Unilever Research Colworth, Sharnbrook, Bedford, United Kingdom aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/9(2004-09-01), 1043-1050 1434-6621 42:9<1043 2004 42 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter