Evaluation of a shorter methionine loading test
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| 024 | 7 | 0 | |a 10.1515/CCLM.2004.207 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/CCLM.2004.207 | ||
| 245 | 0 | 0 | |a Evaluation of a shorter methionine loading test |h [Elektronische Daten] |c [Robert De Jonge, Pieter H. Griffioen, Bertrand van Zelst, R. Montserrate Brouns, Willy Visser, Jan Lindemans] |
| 520 | 3 | |a We validated whether a shorter methionine loading test is as accurate as the original 6-h test in identifying hyperhomocysteinemic patients and investigated determinants of fasting and post-load homocysteine concentration. Plasma homocysteine was determined in EDTA-blood from women with a history of preeclampsia (n=106) after 12 h fasting and 3 and 6 h after an oral methionine load (0.1 g/kg body weight). The 677C > T polymorphism in the methylenetetrahydrofolate reductase (MTHFR) gene, vitamin B6, vitamin B12, folate and creatinine were measured as determinants of homocysteine concentration. Good correlation and agreement between 3-h and 6-h plasma concentration of post-load (r=0.93, Kendall's tau-b=0.85) and Δ (post-load minus the fasting value; r=0.90, Kendall's tau-b=0.79) homocysteine was observed and gross misclassification did not occur after division of 3-h and 6-h homocysteine scores into quartiles. Multiple linear regression revealed MTHFR 677 TT (p=0.01), folate (p=0.04) and vitamin B12 (p=0.06) as determinants of fasting homocysteine concentration; only MTHFR 677TT was related to 3-h (p=0.04) and 6-h (p=0.004) post-load homocysteine concentration. The MTHFR 677TT genotype resulted in > 30% higher fasting and 3-h and 6-h post-load homocysteine concentrations compared to the wildtype CC genotype. This study shows that the 3-h methionine loading test is as good as the 6-h methionine loading test in identifying hyperhomocysteinemic patients. Furthermore, remethylation parameters (MTHFR 677C > T) strongly affect both fasting and post-load homocysteine. | |
| 540 | |a © Walter de Gruyter | ||
| 690 | 7 | |a Medical equipment & techniques |2 nationallicence | |
| 690 | 7 | |a Medical diagnosis |2 nationallicence | |
| 690 | 7 | |a Diseases & disorders |2 nationallicence | |
| 690 | 7 | |a homocysteine |2 nationallicence | |
| 690 | 7 | |a methionine loading test |2 nationallicence | |
| 690 | 7 | |a methylenetetrahydrofolate reductase (MTHFR) |2 nationallicence | |
| 690 | 7 | |a mutation |2 nationallicence | |
| 700 | 1 | |a De Jonge |D Robert |u Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands |4 aut | |
| 700 | 1 | |a Griffioen |D Pieter H. |u Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands |4 aut | |
| 700 | 1 | |a van Zelst |D Bertrand |u Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands |4 aut | |
| 700 | 1 | |a Montserrate Brouns |D R. |u Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands |4 aut | |
| 700 | 1 | |a Visser |D Willy |u Obstetrics and Gynaecology, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands |4 aut | |
| 700 | 1 | |a Lindemans |D Jan |u Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands |4 aut | |
| 773 | 0 | |t Clinical Chemistry and Laboratory Medicine |d Walter de Gruyter |g 42/9(2004-09-01), 1027-1031 |x 1434-6621 |q 42:9<1027 |1 2004 |2 42 |o cclm | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/CCLM.2004.207 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/CCLM.2004.207 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a De Jonge |D Robert |u Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Griffioen |D Pieter H. |u Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a van Zelst |D Bertrand |u Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Montserrate Brouns |D R. |u Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Visser |D Willy |u Obstetrics and Gynaecology, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lindemans |D Jan |u Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Clinical Chemistry and Laboratory Medicine |d Walter de Gruyter |g 42/9(2004-09-01), 1027-1031 |x 1434-6621 |q 42:9<1027 |1 2004 |2 42 |o cclm | ||
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| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-gruyter | ||