caa a22 4500 37890468X CHVBK 20180305123527.0 cr unu---uuuuu 161128e20040901xx s 000 0 eng 10.1515/CCLM.2004.207 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2004.207 Evaluation of a shorter methionine loading test [Elektronische Daten] [Robert De Jonge, Pieter H. Griffioen, Bertrand van Zelst, R. Montserrate Brouns, Willy Visser, Jan Lindemans] We validated whether a shorter methionine loading test is as accurate as the original 6-h test in identifying hyperhomocysteinemic patients and investigated determinants of fasting and post-load homocysteine concentration. Plasma homocysteine was determined in EDTA-blood from women with a history of preeclampsia (n=106) after 12 h fasting and 3 and 6 h after an oral methionine load (0.1 g/kg body weight). The 677C > T polymorphism in the methylenetetrahydrofolate reductase (MTHFR) gene, vitamin B6, vitamin B12, folate and creatinine were measured as determinants of homocysteine concentration. Good correlation and agreement between 3-h and 6-h plasma concentration of post-load (r=0.93, Kendall's tau-b=0.85) and Δ (post-load minus the fasting value; r=0.90, Kendall's tau-b=0.79) homocysteine was observed and gross misclassification did not occur after division of 3-h and 6-h homocysteine scores into quartiles. Multiple linear regression revealed MTHFR 677 TT (p=0.01), folate (p=0.04) and vitamin B12 (p=0.06) as determinants of fasting homocysteine concentration; only MTHFR 677TT was related to 3-h (p=0.04) and 6-h (p=0.004) post-load homocysteine concentration. The MTHFR 677TT genotype resulted in > 30% higher fasting and 3-h and 6-h post-load homocysteine concentrations compared to the wildtype CC genotype. This study shows that the 3-h methionine loading test is as good as the 6-h methionine loading test in identifying hyperhomocysteinemic patients. Furthermore, remethylation parameters (MTHFR 677C > T) strongly affect both fasting and post-load homocysteine. © Walter de Gruyter Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence homocysteine nationallicence methionine loading test nationallicence methylenetetrahydrofolate reductase (MTHFR) nationallicence mutation nationallicence De Jonge Robert Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands aut Griffioen Pieter H. Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands aut van Zelst Bertrand Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands aut Montserrate Brouns R. Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands aut Visser Willy Obstetrics and Gynaecology, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands aut Lindemans Jan Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/9(2004-09-01), 1027-1031 1434-6621 42:9<1027 2004 42 cclm https://doi.org/10.1515/CCLM.2004.207 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2004.207 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- De Jonge Robert Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands aut NATIONALLICENCE 700 1- Griffioen Pieter H. Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands aut NATIONALLICENCE 700 1- van Zelst Bertrand Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands aut NATIONALLICENCE 700 1- Montserrate Brouns R. Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands aut NATIONALLICENCE 700 1- Visser Willy Obstetrics and Gynaecology, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands aut NATIONALLICENCE 700 1- Lindemans Jan Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/9(2004-09-01), 1027-1031 1434-6621 42:9<1027 2004 42 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter